Brent C Credille1, Amelia R Woolums2, Michael W Overton3, David J Hurley3, Steeve Giguère2. 1. Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, United States. Electronic address: bc24@uga.edu. 2. Department of Large Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, United States. 3. Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, United States.
Abstract
OBJECTIVE: To investigate whether expression of genes associated with inflammation and activity of indoleamine-2,3-dioxygenase (IDO) correlated with disease status and prevalence of bacteremia in post-partum dairy cattle with and without acute puerperal metritis (APM). PROCEDURES: Blood was collected from cattle with APM and control cattle matched by parity and days in milk. Leukocytes were isolated and expression of 6 genes was quantified. Activity of IDO was measured in serum with higher performance liquid chromatography (HPLC). RESULTS: The relative expression of IL-1β in cattle with APM was significantly lower than that in controls. IDO activity was not significantly different between bacteremic and non-bacteremic cattle CONCLUSIONS AND CLINICAL RELEVANCE: The expression of IL-1β was lower in cattle with APM. The lower levels of IL-1β expression in PBMCs of cattle with APM suggest impaired inflammatory responses and may contribute to the development of the disease in this population of animals.
OBJECTIVE: To investigate whether expression of genes associated with inflammation and activity of indoleamine-2,3-dioxygenase (IDO) correlated with disease status and prevalence of bacteremia in post-partum dairy cattle with and without acute puerperal metritis (APM). PROCEDURES: Blood was collected from cattle with APM and control cattle matched by parity and days in milk. Leukocytes were isolated and expression of 6 genes was quantified. Activity of IDO was measured in serum with higher performance liquid chromatography (HPLC). RESULTS: The relative expression of IL-1β in cattle with APM was significantly lower than that in controls. IDO activity was not significantly different between bacteremic and non-bacteremic cattle CONCLUSIONS AND CLINICAL RELEVANCE: The expression of IL-1β was lower in cattle with APM. The lower levels of IL-1β expression in PBMCs of cattle with APM suggest impaired inflammatory responses and may contribute to the development of the disease in this population of animals.
Authors: Mariam Raliou; Doulaye Dembélé; Anna Düvel; Philippe Bolifraud; Julie Aubert; Tristan Mary-Huard; Dominique Rocha; François Piumi; Sophie Mockly; Maike Heppelmann; Isabelle Dieuzy-Labaye; Peter Zieger; David G E Smith; Hans-Joachim Schuberth; Iain Martin Sheldon; Olivier Sandra Journal: PLoS One Date: 2019-08-02 Impact factor: 3.240