Kawther Nourelhouda Boukortt1, Nadjia Saidi-Ouahrani2, Boubaker Boukerzaza3, Hadjira Ouhaibi-Djellouli4, Khalida Hachmaoui5, Fatima Zohra Benaissa6, Leila Taleb7, Hayet Drabla-Ouahrani3, Tahria Deba8, Sid Ahmed Ouledhamou5, Nadhera Mehtar4, Abdellah Boudjema4. 1. Laboratoire de Génétique Moléculaire et Cellulaire, Département de Génétique Moléculaire Appliquée, Faculté des sciences de la nature et de la vie, Université des Sciences et de la Technologie d'Oran-Mohamed BOUDIAF-USTOMB-, BP 1505 El M'naouer, 31036 Oran, Algeria. Electronic address: boukortt.kawther@gmail.com. 2. Département de Biotechnologie, Faculté des Sciences de la Nature et de la Vie, Université d'Oran, BP 1524, El M'Naouar, 31000 Oran, Algeria. 3. Service de parodontologie Département de chirurgie dentaire CHU de Sétif, Oran, Algeria. 4. Laboratoire de Génétique Moléculaire et Cellulaire, Département de Génétique Moléculaire Appliquée, Faculté des sciences de la nature et de la vie, Université des Sciences et de la Technologie d'Oran-Mohamed BOUDIAF-USTOMB-, BP 1505 El M'naouer, 31036 Oran, Algeria. 5. Service de parodontologie Département de chirurgie dentaire EPSP d'Oran, Oran, Algeria. 6. Service de parodontologie Département de chirurgie dentaire CHU de Sidi Belabess, Oran, Algeria. 7. Service de parodontologie Département de chirurgie dentaire CHU de Tlemcen, Oran, Algeria. 8. Faculté de médecine d'Oran, université d'Oran, Oran, Algeria.
Abstract
OBJECTIVE: There is strong evidence that genetic as well as environmental factors affect the development of periodontitis. Various studies suggest that genetic polymorphisms of the interleukin-1 (IL-1) genes are associated with an increased risk of developing the pathogenesis. The aim of the present study was to investigate the possible relationship between two polymorphisms of IL-1 gene cluster IL-1B (C+3954T) (rs1143634) and IL-1A (C-889T) (rs1800587) SNPs and the aggressive and chronic periodontitis risk in a case control study in Algerian population. METHODS: 279 subjects were recruited and received a periodontal examination: 128 healthy controls and 151 cases. From cases, 91 patients were having a chronic disease whereas 60 subjects with aggressive form. All these subjects were genotyped for IL-1A (C-889T) and IL-1B (C+3954T) polymorphisms using TaqMan real time PCR technology. Frequencies of IL-1 alleles, genotypes and the haplotypes were also examined. RESULTS: Significant differences were found in the carriage rate of both minor alleles of the IL-1A (C-889T) and IL-1B (C+3954T) polymorphisms of aggressive periodontitis cases compared with healthy controls (OR [95%CI]=1.61 [1.03-2.49], p=0.03), (OR [95%CI]=1.69 [1.09-2.63], p=0.01), respectively. The result did not reach significance with the chronic form. CONCLUSION: The studied polymorphisms of the IL-1 genes appear to be associated with susceptibility to aggressive periodontitis (AgP) in the Algerian population.
OBJECTIVE: There is strong evidence that genetic as well as environmental factors affect the development of periodontitis. Various studies suggest that genetic polymorphisms of the interleukin-1 (IL-1) genes are associated with an increased risk of developing the pathogenesis. The aim of the present study was to investigate the possible relationship between two polymorphisms of IL-1 gene cluster IL-1B (C+3954T) (rs1143634) and IL-1A (C-889T) (rs1800587) SNPs and the aggressive and chronic periodontitis risk in a case control study in Algerian population. METHODS: 279 subjects were recruited and received a periodontal examination: 128 healthy controls and 151 cases. From cases, 91 patients were having a chronic disease whereas 60 subjects with aggressive form. All these subjects were genotyped for IL-1A (C-889T) and IL-1B (C+3954T) polymorphisms using TaqMan real time PCR technology. Frequencies of IL-1 alleles, genotypes and the haplotypes were also examined. RESULTS: Significant differences were found in the carriage rate of both minor alleles of the IL-1A (C-889T) and IL-1B (C+3954T) polymorphisms of aggressive periodontitis cases compared with healthy controls (OR [95%CI]=1.61 [1.03-2.49], p=0.03), (OR [95%CI]=1.69 [1.09-2.63], p=0.01), respectively. The result did not reach significance with the chronic form. CONCLUSION: The studied polymorphisms of the IL-1 genes appear to be associated with susceptibility to aggressive periodontitis (AgP) in the Algerian population.