Ying Han1, Guang-Xu Xu1, Huan Lu1, Dan-Hua Yu1, Yuan Ren2, Li Wang3, Xu-Hong Huang1, Wen-Jing Hou1, Zhen-Hong Wei1, Yuan-Ping Chen1, Yun-Gui Cao4, Rong Zhang1. 1. Department of Obstetrics and Gynecology, Fengxian Hospital, Southern Medical University Shanghai 201499, P. R. China. 2. Obstetrics and Gynecology Hospital, Fudan University Shanghai 200011, P. R. China ; Changzhou Maternal And Child Health Care Hospital 213003 Jiangsu, P. R. China. 3. Changzhou Maternal And Child Health Care Hospital 213003 Jiangsu, P. R. China. 4. Shanghai Jiading Maternal and Child Health Care Hospital Shanghai 201899, P. R. China.
Abstract
OBJECTIVE: This study aimed to characterize the miR-21 and evaluated its clinical significance. METHODS: Total RNA was extracted from 30 pairs of fresh specimens of cervical cancer and normal tissues. The expression levels of the miR-21-3p and miR-21-5p were detected by quantitative reverse transcriptase polymerase chain reaction, with U6 as the internal reference gene. We compared the expression of miR-21-3p and miR-21-5p between study group and control groups, the association between miRNA expression level and clinicopathological factors was investigated. RESULTS: The expression of miR-21-3p and miR-21-5p in HPV positive cervical cancer samples was significantly upregulated compared to that in the paired normal samples (P < 0.05); A multivariate analysis demonstrated that the expression of miR-21 was associated with clinicopathological parameters, including depth of invasion and lymph node metastasis. CONCLUSIONS: MiR-21 upregulation is associated with aggressive progression and poor prognosis in cervical cancer, which suggests that miR-21 might be identified as an independent marker for predicting the clinical outcome of cervical cancer patients.
OBJECTIVE: This study aimed to characterize the miR-21 and evaluated its clinical significance. METHODS: Total RNA was extracted from 30 pairs of fresh specimens of cervical cancer and normal tissues. The expression levels of the miR-21-3p and miR-21-5p were detected by quantitative reverse transcriptase polymerase chain reaction, with U6 as the internal reference gene. We compared the expression of miR-21-3p and miR-21-5p between study group and control groups, the association between miRNA expression level and clinicopathological factors was investigated. RESULTS: The expression of miR-21-3p and miR-21-5p in HPV positive cervical cancer samples was significantly upregulated compared to that in the paired normal samples (P < 0.05); A multivariate analysis demonstrated that the expression of miR-21 was associated with clinicopathological parameters, including depth of invasion and lymph node metastasis. CONCLUSIONS:MiR-21 upregulation is associated with aggressive progression and poor prognosis in cervical cancer, which suggests that miR-21 might be identified as an independent marker for predicting the clinical outcome of cervical cancerpatients.
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