Yan Diao1, Dong Wu2, Zhijun Dai1, Huafeng Kang1, Ziming Wang3, Xijing Wang1. 1. Department of Oncology, Second Affiliated Hospital, Medical School of Xi'an Jiaotong University Xi'an, People's Republic of China. 2. Department of Emergency, Shaanxi Provincial People's Hospital Xi'an, People's Republic of China. 3. Department of Urology, Second Affiliated Hospital, Medical School of Xi'an Jiaotong University Xi'an, People's Republic of China.
Abstract
BACKGROUND: Deregulation of transformer 2β (Tra2β) has been implicated in several cancers. However, the role of Tra2β expression in prostate cancer (PCa) is unclear. Therefore, this study was to investigate the expression of Tra2β in PCa and evaluated its association with clinicopathological variables and prognosis. METHODS: Thirty paired fresh PCa samples were analyzed for Tra2β expression by Western blot analysis. Immunohistochemistry (IHC) assay was performed in 160 PCa samples after radical prostatectomy and adjacent non-cancerous tissues. Tra2β protein expression was divided into high expression group and low expression group by IHC. We also investigated the association of Tra2β expression with clinical and pathologic parameters. Kaplan-Meier plots and Cox proportional hazards regression model were used to analyze the association between Tra2β protein expression and prognosis of PCa patients. Our results showed that Tra2β was significantly upregulated in PCa tissues by western blot and IHC. RESULTS: Our data indicated that high expression of Tra2β was significantly associated with lymph node metastasis (P=0.002), clinical stage (P=0.015), preoperative prostate-specific antigen (P=0.003), Gleason score (P=0.001), and biochemical recurrence (P=0.021). High Tra2β expression was a significant predictor of poor biochemical recurrence free survival and overall survival both in univariate and multivariate analysis. CONCLUSION: We show that Tra2β was significantly upregulated in PCa patients after radical prostatectomy, and multivariate analysis confirmed Tra2β as an independent prognostic factor.
BACKGROUND: Deregulation of transformer 2β (Tra2β) has been implicated in several cancers. However, the role of Tra2β expression in prostate cancer (PCa) is unclear. Therefore, this study was to investigate the expression of Tra2β in PCa and evaluated its association with clinicopathological variables and prognosis. METHODS: Thirty paired fresh PCa samples were analyzed for Tra2β expression by Western blot analysis. Immunohistochemistry (IHC) assay was performed in 160 PCa samples after radical prostatectomy and adjacent non-cancerous tissues. Tra2β protein expression was divided into high expression group and low expression group by IHC. We also investigated the association of Tra2β expression with clinical and pathologic parameters. Kaplan-Meier plots and Cox proportional hazards regression model were used to analyze the association between Tra2β protein expression and prognosis of PCa patients. Our results showed that Tra2β was significantly upregulated in PCa tissues by western blot and IHC. RESULTS: Our data indicated that high expression of Tra2β was significantly associated with lymph node metastasis (P=0.002), clinical stage (P=0.015), preoperative prostate-specific antigen (P=0.003), Gleason score (P=0.001), and biochemical recurrence (P=0.021). High Tra2β expression was a significant predictor of poor biochemical recurrence free survival and overall survival both in univariate and multivariate analysis. CONCLUSION: We show that Tra2β was significantly upregulated in PCa patients after radical prostatectomy, and multivariate analysis confirmed Tra2β as an independent prognostic factor.
Authors: E David Crawford; Charles L Bennett; Gerald L Andriole; Marc B Garnick; Daniel P Petrylak Journal: BJU Int Date: 2013-07-04 Impact factor: 5.588
Authors: Yi Qin Ouyang; Axel zur Hausen; Marzenna Orlowska-Volk; Markus Jäger; Herta Bettendorf; Marc Hirschfeld; Xiao Wen Tong; Elmar Stickeler Journal: Int J Cancer Date: 2011-05-01 Impact factor: 7.396
Authors: Gary A Clawson; Gail L Matters; Ping Xin; Christopher McGovern; Eric Wafula; Claude dePamphilis; Morgan Meckley; Joyce Wong; Luke Stewart; Christopher D'Jamoos; Naomi Altman; Yuka Imamura Kawasawa; Zhen Du; Loren Honaas; Thomas Abraham Journal: PLoS One Date: 2017-09-28 Impact factor: 3.240
Authors: Stefan Vordenbäumen; Ralph Brinks; Patrick Schriek; Angelika Lueking; Jutta G Richter; Petra Budde; Peter Schulz-Knappe; Hans-Dieter Zucht; Johanna Callhoff; Matthias Schneider Journal: Arthritis Res Ther Date: 2020-07-06 Impact factor: 5.156