Literature DB >> 26260597

Monitoring G Protein Activation in Cells with BRET.

Ikuo Masuho1, Kirill A Martemyanov, Nevin A Lambert.   

Abstract

Live-cell assays based on fluorescence and luminescence are now indispensable tools for the study of G protein signaling. Assays based on fluorescence and bioluminescence resonance energy transfer (FRET and BRET) have been particularly valuable for monitoring changes in second messengers, protein-protein interactions, and protein conformation. Here, we describe a BRET assay that monitors the release of free Gβγ dimers after activation of heterotrimers containing Gα subunits from all four G protein subfamilies. This assay provides useful kinetic and pharmacological information with reasonably high throughput using a standard laboratory equipment.

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Year:  2015        PMID： 26260597      PMCID： PMC4879496          DOI： 10.1007/978-1-4939-2914-6_8

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  9 in total

Review 1.  Seven-transmembrane receptors.

Authors:  Kristen L Pierce; Richard T Premont; Robert J Lefkowitz
Journal:  Nat Rev Mol Cell Biol       Date:  2002-09       Impact factor: 94.444

2.  Visualization of G protein betagamma dimers using bimolecular fluorescence complementation demonstrates roles for both beta and gamma in subcellular targeting.

Authors:  Thomas R Hynes; Linnan Tang; Stacy M Mervine; Jonathan L Sabo; Evan A Yost; Peter N Devreotes; Catherine H Berlot
Journal:  J Biol Chem       Date:  2004-05-10       Impact factor: 5.157

Review 3.  Fluorescence/bioluminescence resonance energy transfer techniques to study G-protein-coupled receptor activation and signaling.

Authors:  Martin J Lohse; Susanne Nuber; Carsten Hoffmann
Journal:  Pharmacol Rev       Date:  2012-03-08       Impact factor: 25.468

4.  Regulators of G-protein signaling accelerate GPCR signaling kinetics and govern sensitivity solely by accelerating GTPase activity.

Authors:  Nevin A Lambert; Christopher A Johnston; Steven D Cappell; Sudhakiranmayi Kuravi; Adam J Kimple; Francis S Willard; David P Siderovski
Journal:  Proc Natl Acad Sci U S A       Date:  2010-03-29       Impact factor: 11.205

5.  Macromolecular composition dictates receptor and G protein selectivity of regulator of G protein signaling (RGS) 7 and 9-2 protein complexes in living cells.

Authors:  Ikuo Masuho; Keqiang Xie; Kirill A Martemyanov
Journal:  J Biol Chem       Date:  2013-07-15       Impact factor: 5.157

6.  Mutations in GNAL: a novel cause of craniocervical dystonia.

Authors:  Kishore R Kumar; Katja Lohmann; Ikuo Masuho; Ryosuke Miyamoto; Andreas Ferbert; Thora Lohnau; Meike Kasten; Johann Hagenah; Norbert Brüggemann; Julia Graf; Alexander Münchau; Vladimir S Kostic; Carolyn M Sue; Aloysius R Domingo; Raymond L Rosales; Lilian V Lee; Karen Freimann; Ana Westenberger; Youhei Mukai; Toshitaka Kawarai; Ryuji Kaji; Christine Klein; Kirill A Martemyanov; Alexander Schmidt
Journal:  JAMA Neurol       Date:  2014-04       Impact factor: 18.302

7.  The c-terminus of GRK3 indicates rapid dissociation of G protein heterotrimers.

Authors:  Bettye Hollins; Sudhakiranmayi Kuravi; Gregory J Digby; Nevin A Lambert
Journal:  Cell Signal       Date:  2009-03-01       Impact factor: 4.315

8.  Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate.

Authors:  Mary P Hall; James Unch; Brock F Binkowski; Michael P Valley; Braeden L Butler; Monika G Wood; Paul Otto; Kristopher Zimmerman; Gediminas Vidugiris; Thomas Machleidt; Matthew B Robers; Hélène A Benink; Christopher T Eggers; Michael R Slater; Poncho L Meisenheimer; Dieter H Klaubert; Frank Fan; Lance P Encell; Keith V Wood
Journal:  ACS Chem Biol       Date:  2012-08-30       Impact factor: 5.100

9.  Essential role of the m2R-RGS6-IKACh pathway in controlling intrinsic heart rate variability.

Authors:  Ekaterina Posokhova; David Ng; Aaisha Opel; Ikuo Masuho; Andrew Tinker; Leslie G Biesecker; Kevin Wickman; Kirill A Martemyanov
Journal:  PLoS One       Date:  2013-10-29       Impact factor: 3.240
  9 in total
  30 in total

1.  Distinct profiles of functional discrimination among G proteins determine the actions of G protein-coupled receptors.

Authors:  Ikuo Masuho; Olga Ostrovskaya; Grant M Kramer; Christopher D Jones; Keqiang Xie; Kirill A Martemyanov
Journal:  Sci Signal       Date:  2015-12-01       Impact factor: 8.192

2.  Novel GNB1 mutations disrupt assembly and function of G protein heterotrimers and cause global developmental delay in humans.

Authors:  Katja Lohmann; Ikuo Masuho; Dipak N Patil; Hauke Baumann; Eva Hebert; Sofia Steinrücke; Daniel Trujillano; Nickolas K Skamangas; Valerija Dobricic; Irina Hüning; Gabriele Gillessen-Kaesbach; Ana Westenberger; Dusanka Savic-Pavicevic; Alexander Münchau; Gabriela Oprea; Christine Klein; Arndt Rolfs; Kirill A Martemyanov
Journal:  Hum Mol Genet       Date:  2017-03-15       Impact factor: 6.150

3.  Loss-of-function of Endothelin receptor type A results in Oro-Oto-Cardiac syndrome.

Authors:  Amanda Barone Pritchard; Stanley M Kanai; Bryan Krock; Erica Schindewolf; Jennifer Oliver-Krasinski; Nahla Khalek; Najeah Okashah; Nevin A Lambert; Andre L P Tavares; Elaine Zackai; David E Clouthier
Journal:  Am J Med Genet A       Date:  2020-03-05       Impact factor: 2.802

4.  The differential actions of clozapine and other antipsychotic drugs on the translocation of dopamine D2 receptors to the cell surface.

Authors:  Joseph M Schrader; Craig M Irving; J Christopher Octeau; Joseph A Christian; Timothy J Aballo; Dean J Kareemo; Joseph Conti; Jodi L Camberg; J Robert Lane; Jonathan A Javitch; Abraham Kovoor
Journal:  J Biol Chem       Date:  2019-01-22       Impact factor: 5.157

5.  Dopamine Receptor DAMB Signals via Gq to Mediate Forgetting in Drosophila.

Authors:  Sophie Himmelreich; Ikuo Masuho; Jacob A Berry; Courtney MacMullen; Nickolas K Skamangas; Kirill A Martemyanov; Ronald L Davis
Journal:  Cell Rep       Date:  2017-11-21       Impact factor: 9.423

6.  The orphan receptor GPR139 signals via Gq/11 to oppose opioid effects.

Authors:  Hannah M Stoveken; Stefano Zucca; Ikuo Masuho; Brock Grill; Kirill A Martemyanov
Journal:  J Biol Chem       Date:  2020-06-23       Impact factor: 5.157

7.  Synergistically acting agonists and antagonists of G protein-coupled receptors prevent photoreceptor cell degeneration.

Authors:  Yu Chen; Grazyna Palczewska; Ikuo Masuho; Songqi Gao; Hui Jin; Zhiqian Dong; Linn Gieser; Matthew J Brooks; Philip D Kiser; Timothy S Kern; Kirill A Martemyanov; Anand Swaroop; Krzysztof Palczewski
Journal:  Sci Signal       Date:  2016-07-26       Impact factor: 8.192

8.  GPCR-dependent biasing of GIRK channel signaling dynamics by RGS6 in mouse sinoatrial nodal cells.

Authors:  Allison Anderson; Ikuo Masuho; Ezequiel Marron Fernandez de Velasco; Atsushi Nakano; Lutz Birnbaumer; Kirill A Martemyanov; Kevin Wickman
Journal:  Proc Natl Acad Sci U S A       Date:  2020-06-08       Impact factor: 11.205

9.  Screening of GNAL variants in Brazilian patients with isolated dystonia reveals a novel mutation with partial loss of function.

Authors:  Camila Oliveira Dos Santos; Ikuo Masuho; Francisco Pereira da Silva-Júnior; Egberto Reis Barbosa; Sonia Maria Cesar Azevedo Silva; Vanderci Borges; Henrique Ballalai Ferraz; Maria Sheila Guimarães Rocha; João Carlos Papaterra Limongi; Kirill A Martemyanov; Patricia de Carvalho Aguiar
Journal:  J Neurol       Date:  2016-01-25       Impact factor: 4.849

10.  Rapid kinetic BRET measurements to monitor G protein activation by GPCR and non-GPCR proteins.

Authors:  Marcin Maziarz; Mikel Garcia-Marcos
Journal:  Methods Cell Biol       Date:  2017-09-12       Impact factor: 1.441
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