Doglas Cecchin1, Letícia Cazarotto Pin2, Ana Paula Farina2, Matheus Souza2, Cristina de Mattos Pimenta Vidal2, Yuri Dal Bello2, Caio Cezar Randi Ferraz3, Ana Bedran-Russo4. 1. Department of Restorative Dentistry, Dental School of Universidade de Passo Fundo, Passo Fundo, Rio Grande do Sul, Brazil. Electronic address: dgscecchin@yahoo.com.br. 2. Department of Restorative Dentistry, Dental School of Universidade de Passo Fundo, Passo Fundo, Rio Grande do Sul, Brazil. 3. Department of Restorative Dentistry, Piracicaba Dental School of Universidade Estadual de Campinas, Piracicaba, São Paulo, Brazil. 4. Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, Chicago, Illinois.
Abstract
INTRODUCTION: The aim of this study was to investigate the effects of a proanthocyanidin-rich extract (grape seed extract [GSE]) on the bond strength and stability of the adhesion of fiber posts to the root dentine using 2 adhesive systems: a total-etch and a self-etch adhesive system. METHODS: Single-rooted human teeth were randomly divided into 6 groups: G1 (control), untreated + total-etch adhesive Adper Scotchbond Multi-Purpose (3M ESPE, St Paul, MN); G2, 6.5% GSE for 5 minutes + SB; G3, 10% GSE for 5 minutes + SB; and G4 to G6 groups were similar to previous ones; however, the self-etch adhesive system Clearfil SE Bond (Kuraray, Kurashiki, Japan) was used. Fiber posts were cemented with RelyX ARC (3M ESPE), and the specimens were immediately tested for push out or stored for 12 months. The bond strength means were analyzed by analysis of variance and Games-Howell post hoc tests (α = 0.05). Additionally, matrix metalloproteinase inhibition by 0.65%, 0.065%, and 0.0065% GSE was examined with gelatin zymography. RESULTS: The use of GSE did not affect immediate bond strength to dentin and contributed to preserve the bond strength after 12 months (P < .05) for both adhesives. The bond strength of SB was significantly higher than Clearfil SE Bond (P < .05). Gelatin zymography showed reduced matrix metalloproteinase activity when recombinant enzymes were incubated with both 0.65% and 0.065% GSE, with complete inhibition at the highest concentration. CONCLUSIONS: The pretreatment with GSE can be used as a natural alternative to improve bond strength stability of dentin-adhesive interfaces in root canals.
INTRODUCTION: The aim of this study was to investigate the effects of a proanthocyanidin-rich extract (grape seed extract [GSE]) on the bond strength and stability of the adhesion of fiber posts to the root dentine using 2 adhesive systems: a total-etch and a self-etch adhesive system. METHODS: Single-rooted human teeth were randomly divided into 6 groups: G1 (control), untreated + total-etch adhesive Adper Scotchbond Multi-Purpose (3M ESPE, St Paul, MN); G2, 6.5% GSE for 5 minutes + SB; G3, 10% GSE for 5 minutes + SB; and G4 to G6 groups were similar to previous ones; however, the self-etch adhesive system Clearfil SE Bond (Kuraray, Kurashiki, Japan) was used. Fiber posts were cemented with RelyX ARC (3M ESPE), and the specimens were immediately tested for push out or stored for 12 months. The bond strength means were analyzed by analysis of variance and Games-Howell post hoc tests (α = 0.05). Additionally, matrix metalloproteinase inhibition by 0.65%, 0.065%, and 0.0065% GSE was examined with gelatin zymography. RESULTS: The use of GSE did not affect immediate bond strength to dentin and contributed to preserve the bond strength after 12 months (P < .05) for both adhesives. The bond strength of SB was significantly higher than Clearfil SE Bond (P < .05). Gelatin zymography showed reduced matrix metalloproteinase activity when recombinant enzymes were incubated with both 0.65% and 0.065% GSE, with complete inhibition at the highest concentration. CONCLUSIONS: The pretreatment with GSE can be used as a natural alternative to improve bond strength stability of dentin-adhesive interfaces in root canals.
Authors: Mostafa Yourdkhani; Ariene Arcas Leme-Kraus; Berdan Aydin; Ana Karina Bedran-Russo; Scott R White Journal: Dent Mater Date: 2017-04-06 Impact factor: 5.304
Authors: Ana Beatriz Silva Sousa; Cristina M P Vidal; Ariene Arcas Leme-Kraus; Fernanda C P Pires-de-Souza; Ana K Bedran-Russo Journal: Dent Mater Date: 2016-08-11 Impact factor: 5.304