| Literature DB >> 26256755 |
Pascale R Leroueil, Stassi DiMaggio1, Abigail N Leistra2, Craig D Blanchette3, Christine Orme3, Kumar Sinniah2, Bradford G Orr, Mark M Banaszak Holl.
Abstract
Atomic force microscopy force-pulling experiments have been used to measure the binding forces between folic acid (FA) conjugated poly(amidoamine) (PAMAM) dendrimers and folate binding protein (FBP). The generation 5 (G5) PAMAM conjugates contained an average of 2.7, 4.7, and 7.2 FA per dendrimer. The most probable rupture force was measured to be 83, 201, and 189 pN for G5-FA2.7, G5-FA4.7, and G5-FA7.2, respectively. Folic acid blocking experiments for G5-FA7.2 reduced the frequency of successful binding events and increased the magnitude of the average rupture force to 274 pN. The force data are interpreted as arising from a network of van der Waals and electrostatic interactions that form between FBP and G5 PAMAM dendrimer, resulting in a binding strength far greater than that expected for an interaction between FA and FBP alone.Entities:
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Year: 2015 PMID： 26256755 PMCID： PMC4779365 DOI： 10.1021/acs.jpcb.5b05391
Source DB: PubMed Journal: J Phys Chem B ISSN： 1520-5207 Impact factor: 2.991