Manuel Gómez-Guzmán1, Marta Toral1, Miguel Romero1,2, Rosario Jiménez1,2, Pilar Galindo1, Manuel Sánchez1, María José Zarzuelo1, Mónica Olivares3, Julio Gálvez2,4, Juan Duarte1,2. 1. Departamento de Farmacología, Facultad de Farmacia, Universidad de Granada, Granada, Spain. 2. Instituto de Investigación Biosanitaria de Granada (ibs. GRANADA), Granada, Spain. 3. Laboratorio de Descubrimiento y Preclínica, Departamento de Investigación BIOSEARCH S.A, Granada, Spain. 4. CIBER-ehd, Departamento de Farmacología, CIBM, Universidad de Granada, Armilla (Granada), Spain.
Abstract
SCOPE: The cardiovascular effects of probiotics Lactobacillus fermentum CECT5716 (LC40), or L. coryniformis CECT5711 (K8) plus L. gasseri CECT5714 (LC9) (1:1) in spontaneously hypertensive rats (SHR) were evaluated. METHODS AND RESULTS: Ten Wistar Kyoto rats (WKY) and 30 SHR were randomly assigned to four groups (n = 10): a control WKY group, a control SHR groups, an SHR group treated with LC40, and an SHR treated with K8/LC9 group for 5 weeks (at a dose of 3.3 × 10(10) colony-forming units/day in drinking water). Long-term administration of probiotics reduced systolic blood pressure. The consumption of K8/LC9 mixture significantly reduced the cardiac and renal hypertrophy. Both groups of probiotics reversed the impaired aortic endothelium-dependent relaxation to acetylcholine observed in SHR. They also abolished the increased aortic superoxide levels by reducing the increased toll-like receptor-4 mRNA levels and NADPH oxidase activity found in SHR. K8/LC9 consumption also increased endothelial nitric oxide synthase phosphorylation. Probiotic treatments induced a change in the cecum microbiota of SHR, with higher counts of the Lactobacillus spp. cluster, and lower counts of Bacteriodes spp. and Clostridium spp. CONCLUSION: Probiotics exert cardiovascular protective effects in genetic hypertension related to the improvement of vascular pro-oxidative and pro-inflammatory status.
SCOPE: The cardiovascular effects of probiotics Lactobacillus fermentum CECT5716 (LC40), or L. coryniformis CECT5711 (K8) plus L. gasseri CECT5714 (LC9) (1:1) in spontaneously hypertensiverats (SHR) were evaluated. METHODS AND RESULTS: Ten Wistar Kyoto rats (WKY) and 30 SHR were randomly assigned to four groups (n = 10): a control WKY group, a control SHR groups, an SHR group treated with LC40, and an SHR treated with K8/LC9 group for 5 weeks (at a dose of 3.3 × 10(10) colony-forming units/day in drinking water). Long-term administration of probiotics reduced systolic blood pressure. The consumption of K8/LC9 mixture significantly reduced the cardiac and renal hypertrophy. Both groups of probiotics reversed the impaired aortic endothelium-dependent relaxation to acetylcholine observed in SHR. They also abolished the increased aortic superoxide levels by reducing the increased toll-like receptor-4 mRNA levels and NADPH oxidase activity found in SHR. K8/LC9 consumption also increased endothelial nitric oxide synthase phosphorylation. Probiotic treatments induced a change in the cecum microbiota of SHR, with higher counts of the Lactobacillus spp. cluster, and lower counts of Bacteriodes spp. and Clostridium spp. CONCLUSION: Probiotics exert cardiovascular protective effects in genetic hypertension related to the improvement of vascular pro-oxidative and pro-inflammatory status.
Authors: Iñaki Robles-Vera; Marta Toral; Miguel Romero; Rosario Jiménez; Manuel Sánchez; Francisco Pérez-Vizcaíno; Juan Duarte Journal: Curr Hypertens Rep Date: 2017-04 Impact factor: 5.369
Authors: Sareema Adnan; James W Nelson; Nadim J Ajami; Venugopal R Venna; Joseph F Petrosino; Robert M Bryan; David J Durgan Journal: Physiol Genomics Date: 2016-12-23 Impact factor: 3.107
Authors: Mobin Malik; Tisha M Suboc; Sudhi Tyagi; Nita Salzman; Jingli Wang; Rong Ying; Michael J Tanner; Mamatha Kakarla; John E Baker; Michael E Widlansky Journal: Circ Res Date: 2018-10-12 Impact factor: 17.367