Literature DB >> 26255133

High Resolution Melting-Typing (HRMT) of methicillin-resistant Staphylococcus aureus (MRSA): The new frontier to replace multi-locus sequence typing (MLST) for epidemiological surveillance studies.

Gino Mongelli1, Dafne Bongiorno1, Marilena Agosta1, Sabrina Benvenuto1, Stefania Stefani1, Floriana Campanile2.   

Abstract

We report an implemented molecular-typing-method based on HRMA to detect SNPs within MLST loci, characterizing 100 clinical MRSA and 11 control strains, representative of Italian clones. The results provide solid evidence that HRMT could be a fast, cost-effective and reliable alternative to MLST, for MRSA molecular epidemiology.
Copyright © 2015 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Cost and time reduction; High Resolution Melting Typing (HRMT); Methicillin-resistant Staphylococcus aureus (MRSA); Molecular-epidemiology; Single-nucleotide polymorphisms (SNPs)

Mesh:

Year:  2015        PMID: 26255133     DOI: 10.1016/j.mimet.2015.08.001

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN: 0167-7012            Impact factor:   2.363


  2 in total

1.  Rapid containment of nosocomial transmission of a rare community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) clone, responsible for the Staphylococcal Scalded Skin Syndrome (SSSS).

Authors:  Onofrio Lamanna; Dafne Bongiorno; Lisa Bertoncello; Stefano Grandesso; Sandra Mazzucato; Giovanni Battista Pozzan; Mario Cutrone; Michela Chirico; Flavia Baesso; Pierluigi Brugnaro; Viviana Cafiso; Stefania Stefani; Floriana Campanile
Journal:  Ital J Pediatr       Date:  2017-01-06       Impact factor: 2.638

2.  MeltingPlot, a user-friendly online tool for epidemiological investigation using High Resolution Melting data.

Authors:  Matteo Perini; Gherard Batisti Biffignandi; Domenico Di Carlo; Ajay Ratan Pasala; Aurora Piazza; Simona Panelli; Gian Vincenzo Zuccotti; Francesco Comandatore
Journal:  BMC Bioinformatics       Date:  2021-02-18       Impact factor: 3.169

  2 in total

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