Masoumeh Ghasemi1, Elham Kashani2, Azadeh Fayyaz3, Marzieh Attar3, Majid Shahbazi4. 1. Department of Human Genetics and Medical Cellular & Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran. 2. Department of Obstetrics & Gynecology, Sayyad Shirazi Hospital and Medical Cellular & Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran. 3. Medical Cellular & Molecular Research Center, Talghani Children Hospital, Golestan University of Medical Sciences, Bolv Janbazan, Zip code: 4916668197 Gorgan, Iran. 4. Medical Cellular & Molecular Research Center, Talghani Children Hospital, Golestan University of Medical Sciences, Bolv Janbazan, Zip code: 4916668197 Gorgan, Iran. Electronic address: shahbazimajid@yahoo.co.uk.
Abstract
OBJECTIVE: Preeclampsia is a syndrome that affects 5% of all pregnancies, producing substantial maternal and prenatal morbidity and mortality. Several studies have reported that cytokine genes are associated with the persistence of preeclampsia or the severity of the disease. The aim of this study is to investigate the relationships between the polymorphisms of interleukin-1 alpha-889 (IL-1A) gene and preeclampsia. METHOD: Genomic DNA was extracted from the peripheral blood of 305 patients with preeclampsia and 325 normal controls from Sayyad Shirazi Hospital of Golestan University. Then subjected to SSP-PCR amplification. STATA software and the chi square test were used for statistic calculations. RESULTS: The frequencies of IL-1A -889 genotypes C/C, T/T and C/T in preeclampsia cases were 34.8%, 8.2%, 57% and in controls were 20.9%, 7.6% and 71.3% respectively. There was a significant 1.5 fold excess frequency in genotype C/C in cases (CI=1.44-3.07, OR=2.1, P=0.0001). There was a significant difference in the frequencies of alleles or genotypes in IL-1A promoter regions between patients with preeclampsia and the control group. Turkomans showed the highest frequency of the C allele and Sistanies had the lowest frequency of the C allele in preeclampsia compared to control groups (CI=1.5-3.9, OR=2.48, P=0.0001). CONCLUSION: Our findings suggest that the IL-1A-899C/C genotype and C allele are associated with susceptibility to preeclampsia.
OBJECTIVE:Preeclampsia is a syndrome that affects 5% of all pregnancies, producing substantial maternal and prenatal morbidity and mortality. Several studies have reported that cytokine genes are associated with the persistence of preeclampsia or the severity of the disease. The aim of this study is to investigate the relationships between the polymorphisms of interleukin-1 alpha-889 (IL-1A) gene and preeclampsia. METHOD: Genomic DNA was extracted from the peripheral blood of 305 patients with preeclampsia and 325 normal controls from Sayyad Shirazi Hospital of Golestan University. Then subjected to SSP-PCR amplification. STATA software and the chi square test were used for statistic calculations. RESULTS: The frequencies of IL-1A -889 genotypes C/C, T/T and C/T in preeclampsia cases were 34.8%, 8.2%, 57% and in controls were 20.9%, 7.6% and 71.3% respectively. There was a significant 1.5 fold excess frequency in genotype C/C in cases (CI=1.44-3.07, OR=2.1, P=0.0001). There was a significant difference in the frequencies of alleles or genotypes in IL-1A promoter regions between patients with preeclampsia and the control group. Turkomans showed the highest frequency of the C allele and Sistanies had the lowest frequency of the C allele in preeclampsia compared to control groups (CI=1.5-3.9, OR=2.48, P=0.0001). CONCLUSION: Our findings suggest that the IL-1A-899C/C genotype and C allele are associated with susceptibility to preeclampsia.