L Assis1, L P Milares2, T Almeida2, C Tim3, A Magri2, K R Fernandes2, C Medalha2, A C Muniz Renno2. 1. Department of Bioscience, Federal University of São Paulo, Santos, SP, Brazil. Electronic address: livinha_fisio@yahoo.com.br. 2. Department of Bioscience, Federal University of São Paulo, Santos, SP, Brazil. 3. Department of Physiotherapy, Federal University of São Carlos, São Carlos, SP, Brazil.
Abstract
OBJECTIVE: The aim of this study was to evaluate the effects of an aerobic exercise training and low-level laser therapy (LLLT) (associated or not) on degenerative modifications and inflammatory mediators on the articular cartilage using an experimental model of knee OA. MATERIAL AND METHODS: Fifty male Wistar rats were randomly divided into five groups: control group (CG); knee OA control group (OAC); OA plus exercise training group (OAT); OA plus LLLT group (OAL); OA plus exercise training associated with LLLT group (OATL). The exercise training (treadmill; 16 m/min; 50 min/day) and the laser irradiation (two points-medial and lateral side of the left joint; 24 sessions) started 4 weeks after the surgery, 3 days/week for 8 weeks. RESULTS: The results showed that all treated groups showed (irradiated or not) a better pattern of tissue organization, with less fibrillation and irregularities along the articular surface and chondrocytes organization, a lower degenerative process measured by OARSI score and higher thickness values. Additionally, all treated group showed a reduced expression in IL-1β, caspase-3 and MMP-13 compared to OAC. Moreover, a lower caspase-3 expression was observed in OATL compared to OAL and OAT. CONCLUSION: These results suggest that exercise training and LLLT were effective in preventing cartilage degeneration and modulating inflammatory process induced by knee OA.
OBJECTIVE: The aim of this study was to evaluate the effects of an aerobic exercise training and low-level laser therapy (LLLT) (associated or not) on degenerative modifications and inflammatory mediators on the articular cartilage using an experimental model of knee OA. MATERIAL AND METHODS: Fifty male Wistar rats were randomly divided into five groups: control group (CG); knee OA control group (OAC); OA plus exercise training group (OAT); OA plus LLLT group (OAL); OA plus exercise training associated with LLLT group (OATL). The exercise training (treadmill; 16 m/min; 50 min/day) and the laser irradiation (two points-medial and lateral side of the left joint; 24 sessions) started 4 weeks after the surgery, 3 days/week for 8 weeks. RESULTS: The results showed that all treated groups showed (irradiated or not) a better pattern of tissue organization, with less fibrillation and irregularities along the articular surface and chondrocytes organization, a lower degenerative process measured by OARSI score and higher thickness values. Additionally, all treated group showed a reduced expression in IL-1β, caspase-3 and MMP-13 compared to OAC. Moreover, a lower caspase-3 expression was observed in OATL compared to OAL and OAT. CONCLUSION: These results suggest that exercise training and LLLT were effective in preventing cartilage degeneration and modulating inflammatory process induced by knee OA.
Authors: Tatiane Garcia Stancker; Stella Souza Vieira; Andrey Jorge Serra; Rafael do Nascimento Lima; Regiane Dos Santos Feliciano; José Antônio Silva; Solange Almeida Dos Santos; Marcia Ataize Dos Santos Vieira; Maíra Cecília Brandão Simões; Ernesto Cesar Leal-Junior; Paulo de Tarso Camillo de Carvalho Journal: Lasers Med Sci Date: 2018-03-08 Impact factor: 3.161
Authors: Vanessa Lima Cavalcante de Oliveira; José Antonio Silva; Andrey Jorge Serra; Rodney Capp Pallotta; Evela Aparecida Pereira da Silva; Anna Cristina de Farias Marques; Regiane Dos Santos Feliciano; Rodrigo Labat Marcos; Ernesto Cesar Pinto Leal-Junior; Paulo de Tarso Camillo de Carvalho Journal: Lasers Med Sci Date: 2016-10-10 Impact factor: 3.161