| Literature DB >> 26253974 |
Xiaoyu Li1, Shiqing Ma2, Chengqi Yi3.
Abstract
Pseudouridine (Ψ) is the most abundant posttranscriptional RNA modification; yet little is known about its prevalence and function in messenger RNA, mostly due to the challenges in the transcriptome-wide detection of Ψ. Here, we report CeU-Seq-a selective chemical labeling and pull-down method for the comprehensive analysis of transcriptome-wide pseudouridylation; this sequencing method will hopefully pave the way for functional studies of Ψ-mediated biological regulation in the future.Entities:
Keywords: Chemical pull down; Pseudouridine; Selective labeling; Single-base resolution; Transcriptome-wide profiling; Whole-genome sequencing; mRNA modification
Mesh：
Substances：
Year: 2015 PMID： 26253974 DOI： 10.1016/bs.mie.2015.03.010
Source DB: PubMed Journal: Methods Enzymol ISSN： 0076-6879 Impact factor: 1.600