Hai Fei You1, Jing Zhi Zhao2, Yu Jia Zhai3, Lei Yin2, Chao Pang2, Xin Ping Luo4, Ming Zhang4, Jin Jin Wang5, Lin Lin Li6, Yan Wang6, Qian Wang6, Bing Yuan Wang6, Yong Cheng Ren6, Dong Sheng Hu4. 1. Department of Blood Transfusion, Xijing Hospital, The Fourth Military Medical University, Xi'an 710023, Shannxi, China; Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, Henan, China. 2. Military Hospital of Henan Province, Zhengzhou 450003, Henan, China. 3. Department of Public Health Surveillance & Advisory, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, Zhejiang, China. 4. Shenzhen University School of Medicine, Shenzhen 518060, Guangdong, China. 5. Discipline of Public Health and Preventive Medicine, Center of Preventive Medicine Research and Assessment, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, Henan, China. 6. Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, Henan, China.
Abstract
OBJECTIVE: To investigate the association between low-density lipoprotein receptor-related protein 5 (LRP5) variants (rs12363572 and rs4930588) and type 2 diabetes mellitus (T2DM) in Han Chinese. METHODS: A total of 1842 T2DM cases (507 newly diagnosed cases and 1335 previously diagnosed cases) and 7777 controls were included in this case-control study. PCR-RFLP was conducted to detect the genotype of the two single nucleotide polymorphisms (SNPs). Odds ratios (ORs) and 95% confidence intervals (95% CIs) were calculated to describe the strength of the association by logistic regression. RESULTS: In the study subjects, neither rs12363572 nor rs4930588 was significantly associated with T2DM, even after adjusting for relevant covariates. When stratified by body mass index (BMI), the two SNPs were also not associated with T2DM. Among the 3 common haplotypes, only haplotype TT was associated with reduced risk of T2DM (OR 0.820, 95% CI 0.732-0.919). In addition, rs12363572 was associated with BMI (P<0.001) and rs4930588 was associated with triglyceride levels (P=0.043) in 507 newly diagnosed T2DM cases but not in healthy controls. CONCLUSION: No LRP5 variant was found to be associated with T2DM in Han Chinese, but haplotype TT was found to be associated with T2DM.
OBJECTIVE: To investigate the association between low-density lipoprotein receptor-related protein 5 (LRP5) variants (rs12363572 and rs4930588) and type 2 diabetes mellitus (T2DM) in Han Chinese. METHODS: A total of 1842 T2DM cases (507 newly diagnosed cases and 1335 previously diagnosed cases) and 7777 controls were included in this case-control study. PCR-RFLP was conducted to detect the genotype of the two single nucleotide polymorphisms (SNPs). Odds ratios (ORs) and 95% confidence intervals (95% CIs) were calculated to describe the strength of the association by logistic regression. RESULTS: In the study subjects, neither rs12363572 nor rs4930588 was significantly associated with T2DM, even after adjusting for relevant covariates. When stratified by body mass index (BMI), the two SNPs were also not associated with T2DM. Among the 3 common haplotypes, only haplotype TT was associated with reduced risk of T2DM (OR 0.820, 95% CI 0.732-0.919). In addition, rs12363572 was associated with BMI (P<0.001) and rs4930588 was associated with triglyceride levels (P=0.043) in 507 newly diagnosed T2DM cases but not in healthy controls. CONCLUSION: No LRP5 variant was found to be associated with T2DM in Han Chinese, but haplotype TT was found to be associated with T2DM.