| Literature DB >> 26247304 |
Jian Wei He1, Raymond Yang, Ting Zhou, Greg J Boland, Peter M Scott, Genevieve S Bondy.
Abstract
In an investigation of deoxynivalenol (DON)-transformation products by Devosia mutans 17-2-E-8, the major product was identified as 3-epi-DON. This DON-transformation product was analysed by liquid chromatography and identified by congruent retention time and UV/Vis spectrum, as well as mass spectrometric data. Nuclear magnetic resonance (NMR) experiments including correlation spectroscopy (COSY), heteronuclear single quantum coherence (HSQC) and nuclear overhauser effect (NOE) were conducted for structural characterisation of 3-epi-DON. High-speed counter-current chromatography (HSCCC) was applied to scale up the separation of 3-epi-DON from DON in a D. mutans 17-2-E-8 culture. From the culture where 100 mg DON was applied, 56 mg of 3-epi-DON (purity of 96.8%) was obtained from the HSCCC. The purified 3-epi-DON will be used for toxicological characterisation studies of this chemical.Entities:
Keywords: 3-epi-deoxynivalenol (3-epi-DON); Deoxynivalenol (DON); Devosia mutans; bacterial culture; correlation spectroscopy (COSY); heteronuclear single quantum coherence (HSQC); high-speed counter-current chromatography (HSCCC); liquid chromatography-mass spectrometry (LC-MS); nuclear magnetic resonance (NMR); nuclear overhauser effect (NOE)
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Year: 2015 PMID： 26247304 DOI： 10.1080/19440049.2015.1072771
Source DB: PubMed Journal: Food Addit Contam Part A Chem Anal Control Expo Risk Assess ISSN： 1944-0057