Literature DB >> 26246353

Sendai Virus-Based Reprogramming of Mesenchymal Stromal/Stem Cells from Umbilical Cord Wharton's Jelly into Induced Pluripotent Stem Cells.

Cristian Miere1, Liani Devito1, Dusko Ilic1,2.   

Abstract

In an attempt to bring pluripotent stem cell biology closer to reaching its full potential, many groups have focused on improving reprogramming protocols over the past several years. The episomal modified Sendai virus-based vector has emerged as one of the most practical ones. Here we describe reprogramming of mesenchymal stromal/stem cells (MSC) derived from umbilical cord Wharton's Jelly into induced pluripotent stem cells (iPSC) using genome non-integrating Sendai virus-based vectors. The detailed protocols of iPSC colony cryopreservation (vitrification) and adaption to feeder-free culture conditions are also included.

Entities:  

Keywords:  Adaption to feeder-free culture conditions; MSC; Reprogramming; Sendai virus; Umbilical cord; Vitrification; iPSC

Mesh：

Substances：

Year:  2016        PMID： 26246353     DOI： 10.1007/7651_2014_163

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

1.  A circular RNA map for human induced pluripotent stem cells of foetal origin.

Authors:  Mario Barilani; Alessandro Cherubini; Valeria Peli; Francesca Polveraccio; Valentina Bollati; Federica Guffanti; Alessandro Del Gobbo; Cristiana Lavazza; Silvia Giovanelli; Nicola Elvassore; Lorenza Lazzari
Journal:  EBioMedicine       Date:  2020-06-20       Impact factor: 8.143

2.  Comparison of human isogeneic Wharton's jelly MSCs and iPSC-derived MSCs reveals differentiation-dependent metabolic responses to IFNG stimulation.

Authors:  Liani Devito; Michail E Klontzas; Aleksandra Cvoro; Antonio Galleu; Marisa Simon; Carl Hobbs; Francesco Dazzi; Athanasios Mantalaris; Yacoub Khalaf; Dusko Ilic
Journal:  Cell Death Dis       Date:  2019-03-20       Impact factor: 8.469

3.  Umbilical Cord Tissue as a Source of Young Cells for the Derivation of Induced Pluripotent Stem Cells Using Non-Integrating Episomal Vectors and Feeder-Free Conditions.

Authors:  Aisha Mohamed; Theresa Chow; Jennifer Whiteley; Amanda Fantin; Kersti Sorra; Ryan Hicks; Ian M Rogers
Journal:  Cells       Date:  2020-12-31       Impact factor: 6.600

Review 4.  Young at Heart: Pioneering Approaches to Model Nonischaemic Cardiomyopathy with Induced Pluripotent Stem Cells.

Authors:  Aoife Gowran; Marco Rasponi; Roberta Visone; Patrizia Nigro; Gianluca L Perrucci; Stefano Righetti; Marco Zanobini; Giulio Pompilio
Journal:  Stem Cells Int       Date:  2016-03-24       Impact factor: 5.443

5.  Induced pluripotent stem cell line from an atopic dermatitis patient heterozygous for c.2282del4 mutation in filaggrin: KCLi001-A.

Authors:  Liani Devito; Matthew Donne; Nikola Kolundzic; Preeti Khurana; Carl Hobbs; Gabriel Kaddour; Sandrine Dubrac; Robert Gruber; Matthias Schmuth; Thea Mauro; Dusko Ilic
Journal:  Stem Cell Res       Date:  2018-07-25       Impact factor: 2.020
  5 in total

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