Santwana Mantri1, E Venkata Rao2, Pradeep Kumar Jena3, Prakash Chandra Mohapatra4. 1. Department of Biochemistry, S.C.B. Medical College, Mangalabag, Cuttack, Odisha, 751003, India. santonam_mantri@rediffmail.com. 2. Department of Community Medicine, Institute of Medical Sciences & SUM Hospital, Bhubaneswar, Orissa, India. 3. Department of Pediatric Surgery, SVP Post Graduate Institute of Pediatrics, S.C.B. Medical College, Cuttack, Orissa, India. 4. Department of Biochemistry, S.C.B. Medical College, Mangalabag, Cuttack, Odisha, 751003, India.
Abstract
OBJECTIVE: To characterize the primitive stem cell content of cord blood with regard to neonatal parameters. METHODS: In this cross-sectional study, CD34+ and CD90+ cells content were enumerated by flow-cytometry method. Their associations with various neonatal parameters like birth weight, gender, gestational age and mode of delivery were analyzed by univariate analysis. Multivariable linear regression model was then developed to further explain the effect of neonatal factors on these primitive cell counts. RESULTS: From a total of 106 recruited subjects, gender of the neonate did not have any influence on the expression of these proteins (CD34 and CD90) of cord blood stem cells or progenitors. Multi variable linear regression analysis using CD34+ and CD90+ cell counts as dependent variables revealed that birth weight and the mode of delivery were significant predictors of these cell counts. CONCLUSIONS: The present study suggests that birth weight and mode of delivery of the neonates influences cord blood stem cell yield.
OBJECTIVE: To characterize the primitive stem cell content of cord blood with regard to neonatal parameters. METHODS: In this cross-sectional study, CD34+ and CD90+ cells content were enumerated by flow-cytometry method. Their associations with various neonatal parameters like birth weight, gender, gestational age and mode of delivery were analyzed by univariate analysis. Multivariable linear regression model was then developed to further explain the effect of neonatal factors on these primitive cell counts. RESULTS: From a total of 106 recruited subjects, gender of the neonate did not have any influence on the expression of these proteins (CD34 and CD90) of cord blood stem cells or progenitors. Multi variable linear regression analysis using CD34+ and CD90+ cell counts as dependent variables revealed that birth weight and the mode of delivery were significant predictors of these cell counts. CONCLUSIONS: The present study suggests that birth weight and mode of delivery of the neonates influences cord blood stem cell yield.
Authors: Mitchell S Cairo; Elizabeth L Wagner; John Fraser; Geoff Cohen; Carmella van de Ven; Shelly L Carter; Nancy A Kernan; Joanne Kurtzberg Journal: Transfusion Date: 2005-06 Impact factor: 3.157