Literature DB >> 2624316

A procedure for eliminating interferences in the lowry method of protein determination.

F Rodríguez-Vico1, M Martínez-Cayuela, E García-Peregrín, H Ramírez.   

Abstract

A modification of the Lowry assay for the quantitative protein measurement in the presence of interfering materials has been developed. The method is based on a precipitation with a single-phase hexane:isopropanol solvent system and later resuspension of protein pellets with sodium dodecyl sulfate and deoxycholate. The new procedure eliminates the interference caused by Triton X-100, phospholipids, or dithiothreitol providing yields higher than 95% and seems to be especially suitable for protein determination on membrane preparations in samples with small volumes and/or very low protein concentrations.

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Year:  1989        PMID： 2624316     DOI： 10.1016/0003-2697(89)90479-x

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  8 in total

1.  Crystallization and preliminary crystallographic studies of an active-site mutant hydantoin racemase from Sinorhizobium meliloti CECT4114.

Authors:  Sergio Martínez-Rodríguez; Luis Antonio González-Ramírez; Josefa María Clemente-Jiménez; Felipe Rodríguez-Vico; Francisco Javier Las Heras-Vázquez; Jose Antonio Gavira; Juan Ma García-Ruiz
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2007-12-20

2.  Evidence for masking of brown adipose tissue mitochondrial GDP-binding sites in response to fasting in rats made obese by dietary manipulation. Effects of reversion to standard diet.

Authors:  P Puigserver; I Lladó; A Palou; M Gianotti
Journal:  Biochem J       Date:  1991-10-15       Impact factor: 3.857

3.  Time-resolved spectroscopy of mitochondria, cells and tissues under normal and pathological conditions.

Authors:  B Beauvoit; B Chance
Journal:  Mol Cell Biochem       Date:  1998-07       Impact factor: 3.396

4.  N-Cyclo-N'-(4-Dimethylamino-alpha-Naphthyl)Carbodiimide Inhibits Membrane-Bound and Partially Purified Tonoplast ATPase from Maize Roots.

Authors:  D Brauer; S I Tu
Journal:  Plant Physiol       Date:  1991-03       Impact factor: 8.340

5.  Site-directed mutagenesis indicates an important role of cysteines 76 and 181 in the catalysis of hydantoin racemase from Sinorhizobium meliloti.

Authors:  Sergio Martínez-Rodríguez; Montserrat Andújar-Sánchez; Jose L Neira; Josefa M Clemente-Jiménez; Vicente Jara-Pérez; Felipe Rodríguez-Vico; Francisco J Las Heras-Vázquez
Journal:  Protein Sci       Date:  2006-12       Impact factor: 6.725

6.  Kinetic probes for inter-domain co-operation in human somatic angiotensin-converting enzyme.

Authors:  Olga E Skirgello; Peter V Binevski; Vladimir F Pozdnev; Olga A Kost
Journal:  Biochem J       Date:  2005-11-01       Impact factor: 3.857

7.  Lovastatin increases arachidonic acid levels and stimulates thromboxane synthesis in human liver and monocytic cell lines.

Authors:  N Hrboticky; L Tang; B Zimmer; I Lux; P C Weber
Journal:  J Clin Invest       Date:  1994-01       Impact factor: 14.808

8.  Transmembrane movement of a water-soluble analogue of mannosylphosphoryldolichol is mediated by an endoplasmic reticulum protein.

Authors:  J S Rush; C J Waechter
Journal:  J Cell Biol       Date:  1995-08       Impact factor: 10.539
  8 in total

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