Tinglin Huang1, Lin Guo2, Haihan Zhang3, Junfeng Su3, Gang Wen3, Kai Zhang3. 1. School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Yanta Road 13, Xi'an, Shaanxi 710055, PR China. Electronic address: huangtinglin@xauat.edu.cn. 2. School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Yanta Road 13, Xi'an, Shaanxi 710055, PR China; School of Environment and Planning, Liaocheng University, Liaocheng, Shandong 252000, PR China. 3. School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Yanta Road 13, Xi'an, Shaanxi 710055, PR China.
Abstract
An aerobic denitrifier, identified as Pseudomonas stutzeri strain ZF31, was isolated from the Zhoucun drinking-water reservoir. Strain ZF31 removed 97% of nitrate nitrogen after 16h, without nitrite accumulation. Sequence amplification indicated the presence of the denitrification genes napA, nirS, norB, and nosZ. Nitrogen balance analysis revealed that approximately 75% of the initial nitrogen was removed as gas products. Response surface methodology (RSM) experiments showed that maximum removal of total nitrogen (TN) occurred at pH 8.23, a C/N ratio of 6.68, temperature of 27.72°C, and with shaking at 54.15rpm. The TN removal rate at low C/N ratio (i.e., 3) and low temperature (i.e., 10°C) was 73.30% and 60.08%, respectively. These results suggest that strain ZF31 has potential applications for the bioremediation of slightly polluted drinking-water reservoirs.
An aerobic denitrifier, identified as Pseudomonas stutzeri strain ZF31, was isolated from the Zhoucunn class="Chemical">drinking-water reservoir. Strain ZF31 removed 97% of nitratenitrogen after 16h, without nitrite accumulation. Sequence amplification indicated the presence of the denitrification genes napA, nirS, norB, and nosZ. Nitrogen balance analysis revealed that approximately 75% of the initial nitrogen was removed as gas products. Response surface methodology (RSM) experiments showed that maximum removal of total nitrogen (TN) occurred at pH 8.23, a C/N ratio of 6.68, temperature of 27.72°C, and with shaking at 54.15rpm. The TN removal rate at low C/N ratio (i.e., 3) and low temperature (i.e., 10°C) was 73.30% and 60.08%, respectively. These results suggest that strain ZF31 has potential applications for the bioremediation of slightly polluted drinking-water reservoirs.
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