| Literature DB >> 26241840 |
Tinglin Huang1, Lin Guo2, Haihan Zhang3, Junfeng Su3, Gang Wen3, Kai Zhang3.
Abstract
An aerobic denitrifier, identified as Pseudomonas stutzeri strain ZF31, was isolated from the Zhoucun drinking-water reservoir. Strain ZF31 removed 97% of nitrate nitrogen after 16h, without nitrite accumulation. Sequence amplification indicated the presence of the denitrification genes napA, nirS, norB, and nosZ. Nitrogen balance analysis revealed that approximately 75% of the initial nitrogen was removed as gas products. Response surface methodology (RSM) experiments showed that maximum removal of total nitrogen (TN) occurred at pH 8.23, a C/N ratio of 6.68, temperature of 27.72°C, and with shaking at 54.15rpm. The TN removal rate at low C/N ratio (i.e., 3) and low temperature (i.e., 10°C) was 73.30% and 60.08%, respectively. These results suggest that strain ZF31 has potential applications for the bioremediation of slightly polluted drinking-water reservoirs.Entities:
Keywords: Aerobic denitrifier; Nitrogen removal; Pseudomonas stutzeri; Reservoir; Response surface methodology
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Year: 2015 PMID： 26241840 DOI： 10.1016/j.biortech.2015.07.059
Source DB: PubMed Journal: Bioresour Technol ISSN： 0960-8524 Impact factor: 9.642