Y Chen1, T Wang2, M Guan3, W Zhao4, F-K-L Leung5, H Pan6, X Cao7, X E Guo8, W W Lu9. 1. Department of Orthopaedics and Traumatology, The University of Hong Kong, Hong Kong; Bone Bioengineering Laboratory, Department of Biomedical Engineering, Columbia University, New York, USA. Electronic address: cy003@hku.hk. 2. Center for Human Tissue and Organs Degeneration and Shenzhen Key Laboratory of Marine Biomedical Materials, Shenzhen Institutes of Advanced Technology, CAS, China. Electronic address: wangting@siat.ac.cn. 3. Center for Human Tissue and Organs Degeneration and Shenzhen Key Laboratory of Marine Biomedical Materials, Shenzhen Institutes of Advanced Technology, CAS, China. Electronic address: min.guan@siat.ac.cn. 4. Department of Orthopaedics and Traumatology, The University of Hong Kong, Hong Kong. Electronic address: zhaoww@hku.hk. 5. Department of Orthopaedics and Traumatology, The University of Hong Kong, Hong Kong; Shenzhen Key Laboratory for Innovative Technology in Orthopaedic Trauma, The University of Hong Kong Shenzhen Hospital, China. Electronic address: klleunga@hkucc.hku.hk. 6. Center for Human Tissue and Organs Degeneration and Shenzhen Key Laboratory of Marine Biomedical Materials, Shenzhen Institutes of Advanced Technology, CAS, China. Electronic address: hb.pan@siat.ac.cn. 7. Center for Human Tissue and Organs Degeneration and Shenzhen Key Laboratory of Marine Biomedical Materials, Shenzhen Institutes of Advanced Technology, CAS, China; Department of Orthopaedic Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: xcao11@jhmi.edu. 8. Center for Human Tissue and Organs Degeneration and Shenzhen Key Laboratory of Marine Biomedical Materials, Shenzhen Institutes of Advanced Technology, CAS, China; Bone Bioengineering Laboratory, Department of Biomedical Engineering, Columbia University, New York, USA. Electronic address: exg1@columbia.edu. 9. Department of Orthopaedics and Traumatology, The University of Hong Kong, Hong Kong; Center for Human Tissue and Organs Degeneration and Shenzhen Key Laboratory of Marine Biomedical Materials, Shenzhen Institutes of Advanced Technology, CAS, China; Shenzhen Key Laboratory for Innovative Technology in Orthopaedic Trauma, The University of Hong Kong Shenzhen Hospital, China. Electronic address: wwlu@hku.hk.
Abstract
OBJECTIVE: To investigate changes in bone structure, turnover, and articular cartilage localized in subchondral bone cyst (SBC) regions associated with knee osteoarthritis (OA). METHODS: Tibial plateaus (n = 97) were collected from knee OA patients during total knee arthroplasty (TKA). SBCs were identified using micro-computed tomography, and the specimens were divided into non-cyst (n = 25) and bone cyst (n = 72) groups. Microstructure of subchondral bone was assessed using bone volume fraction (BV/TV), trabecular number (Tb.N), structure model index (SMI) and bone mineral density (BMD). In bone cyst group, the cyst subregion, which contained at least one cyst, and the peri-cyst subregion, which contained no cysts, were further selected for microstructure analysis. Articular cartilage damage was estimated using the Osteoarthritis Research Society International (OARSI) score. The numbers of TRAP(+) osteoclasts, Osterix(+) osteoprogenitors, Osteocalcin(+) osteoblasts and expression of SOX9 were evaluated by immunohistochemistry. RESULTS: Bone cyst group presented higher BV/TV, Tb.N and SMI at subchondral bone than non-cyst group. Furthermore, cyst subregion displayed increased BV/TV and Tb.N but lower BMD and SMI than peri-cyst subregion. Histology revealed a higher OARSI score in bone cyst group. SBC exhibited a weak relationship with BV/TV, etc. The numbers of TRAP(+) osteoclasts, Osterix(+) osteoprogenitors, Osteocalcin(+) osteoblasts and expression of SOX9, were higher in bone cyst group. CONCLUSION: SBCs within knee OA are characterized by focally increased bone turnover, altered bone structure and more severe articular cartilage damage. The increased bone turnover possibly contributes to altered bone structure localized in SBC areas, and thus aggravates articular cartilage degeneration.
OBJECTIVE: To investigate changes in bone structure, turnover, and articular cartilage localized in subchondral bone cyst (SBC) regions associated with knee osteoarthritis (OA). METHODS: Tibial plateaus (n = 97) were collected from knee OA patients during total knee arthroplasty (TKA). SBCs were identified using micro-computed tomography, and the specimens were divided into non-cyst (n = 25) and bone cyst (n = 72) groups. Microstructure of subchondral bone was assessed using bone volume fraction (BV/TV), trabecular number (Tb.N), structure model index (SMI) and bone mineral density (BMD). In bone cyst group, the cyst subregion, which contained at least one cyst, and the peri-cyst subregion, which contained no cysts, were further selected for microstructure analysis. Articular cartilage damage was estimated using the Osteoarthritis Research Society International (OARSI) score. The numbers of TRAP(+) osteoclasts, Osterix(+) osteoprogenitors, Osteocalcin(+) osteoblasts and expression of SOX9 were evaluated by immunohistochemistry. RESULTS: Bone cyst group presented higher BV/TV, Tb.N and SMI at subchondral bone than non-cyst group. Furthermore, cyst subregion displayed increased BV/TV and Tb.N but lower BMD and SMI than peri-cyst subregion. Histology revealed a higher OARSI score in bone cyst group. SBC exhibited a weak relationship with BV/TV, etc. The numbers of TRAP(+) osteoclasts, Osterix(+) osteoprogenitors, Osteocalcin(+) osteoblasts and expression of SOX9, were higher in bone cyst group. CONCLUSION: SBCs within knee OA are characterized by focally increased bone turnover, altered bone structure and more severe articular cartilage damage. The increased bone turnover possibly contributes to altered bone structure localized in SBC areas, and thus aggravates articular cartilage degeneration.
Authors: Jenny Y Zhou; Jonathan H Wong; Zachary T Berman; Alecio F Lombardi; Eric Y Chang; Annette von Drygalski Journal: Haemophilia Date: 2021-09-19 Impact factor: 4.263
Authors: A Nakasone; Y Guang; A Wise; L Kim; J Babbin; S Rathod; A J Mitchell; L C Gerstenfeld; E F Morgan Journal: Osteoarthritis Cartilage Date: 2022-05-13 Impact factor: 7.507
Authors: Yan Chen; Yong-Can Huang; Chun Hoi Yan; Kwong Yuen Chiu; Qingjun Wei; Jingmin Zhao; X Edward Guo; Frankie Leung; William W Lu Journal: Bone Res Date: 2017-11-07 Impact factor: 13.567
Authors: Nicholas Elena; Brittany M Woodall; Katie Lee; Patrick J McGahan; Neil P Pathare; Edward C Shin; James L Chen Journal: Arthrosc Tech Date: 2018-10-15
Authors: Clara Sanjurjo-Rodriguez; Thomas G Baboolal; Agata N Burska; Frederique Ponchel; Jehan J El-Jawhari; Hemant Pandit; Dennis McGonagle; Elena Jones Journal: Sci Rep Date: 2019-06-27 Impact factor: 4.379