Literature DB >> 26240414

Deep Mutational Scanning: A Highly Parallel Method to Measure the Effects of Mutation on Protein Function.

Lea M Starita1, Stanley Fields2.   

Abstract

Deep mutational scanning is a method that makes use of next-generation sequencing technology to measure in a single experiment the activity of 10(5) or more unique variants of a protein. Because of this depth of mutational coverage, this strategy provides data that can be analyzed to reveal many protein properties. Deep mutational scanning approaches are particularly amenable to being performed in Saccharomyces cerevisiae, given the extensive toolkit of reagents and technologies available for this organism.
© 2015 Cold Spring Harbor Laboratory Press.

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Year:  2015        PMID： 26240414     DOI： 10.1101/pdb.top077503

Source DB:  PubMed          Journal:  Cold Spring Harb Protoc        ISSN： 1559-6095


  11 in total

1.  Variant Interpretation: Functional Assays to the Rescue.

Authors:  Lea M Starita; Nadav Ahituv; Maitreya J Dunham; Jacob O Kitzman; Frederick P Roth; Georg Seelig; Jay Shendure; Douglas M Fowler
Journal:  Am J Hum Genet       Date:  2017-09-07       Impact factor: 11.025

2.  Evolution of a designed protein assembly encapsulating its own RNA genome.

Authors:  Gabriel L Butterfield; Marc J Lajoie; Heather H Gustafson; Drew L Sellers; Una Nattermann; Daniel Ellis; Jacob B Bale; Sharon Ke; Garreck H Lenz; Angelica Yehdego; Rashmi Ravichandran; Suzie H Pun; Neil P King; David Baker
Journal:  Nature       Date:  2017-12-13       Impact factor: 49.962

3.  Circular permutation profiling by deep sequencing libraries created using transposon mutagenesis.

Authors:  Joshua T Atkinson; Alicia M Jones; Quan Zhou; Jonathan J Silberg
Journal:  Nucleic Acids Res       Date:  2018-07-27       Impact factor: 16.971

4.  Recent Advances in Machine Learning Variant Effect Prediction Tools for Protein Engineering.

Authors:  Jesse Horne; Diwakar Shukla
Journal:  Ind Eng Chem Res       Date:  2022-04-06       Impact factor: 4.326

5.  Affinity Maturation of a Cyclic Peptide Handle for Therapeutic Antibodies Using Deep Mutational Scanning.

Authors:  Martijn van Rosmalen; Brian M G Janssen; Natalie M Hendrikse; Ardjan J van der Linden; Pascal A Pieters; Dave Wanders; Tom F A de Greef; Maarten Merkx
Journal:  J Biol Chem       Date:  2016-12-14       Impact factor: 5.157

6.  A statistical framework for analyzing deep mutational scanning data.

Authors:  Alan F Rubin; Hannah Gelman; Nathan Lucas; Sandra M Bajjalieh; Anthony T Papenfuss; Terence P Speed; Douglas M Fowler
Journal:  Genome Biol       Date:  2017-08-07       Impact factor: 13.583

7.  ZMPSTE24 missense mutations that cause progeroid diseases decrease prelamin A cleavage activity and/or protein stability.

Authors:  Eric D Spear; Erh-Ting Hsu; Laiyin Nie; Elisabeth P Carpenter; Christine A Hrycyna; Susan Michaelis
Journal:  Dis Model Mech       Date:  2018-07-13       Impact factor: 5.758

8.  GeneORator: An Effective Strategy for Navigating Protein Sequence Space More Efficiently through Boolean OR-Type DNA Libraries.

Authors:  Andrew Currin; Jane Kwok; Joanna C Sadler; Elizabeth L Bell; Neil Swainston; Maria Ababi; Philip Day; Nicholas J Turner; Douglas B Kell
Journal:  ACS Synth Biol       Date:  2019-06-07       Impact factor: 5.110

9.  Protein design-scapes generated by microfluidic DNA assembly elucidate domain coupling in the bacterial histidine kinase CpxA.

Authors:  Iain C Clark; Bruk Mensa; Christopher J Ochs; Nathan W Schmidt; Marco Mravic; Francisco J Quintana; William F DeGrado; Adam R Abate
Journal:  Proc Natl Acad Sci U S A       Date:  2021-03-23       Impact factor: 12.779

10.  Acquired Resistance to KRASG12C Inhibition in Cancer.

Authors:  Mark M Awad; Shengwu Liu; Igor I Rybkin; Kathryn C Arbour; Julien Dilly; Viola W Zhu; Melissa L Johnson; Rebecca S Heist; Tejas Patil; Gregory J Riely; Joseph O Jacobson; Xiaoping Yang; Nicole S Persky; David E Root; Kristen E Lowder; Hanrong Feng; Shannon S Zhang; Kevin M Haigis; Yin P Hung; Lynette M Sholl; Brian M Wolpin; Julie Wiese; Jason Christiansen; Jessica Lee; Alexa B Schrock; Lee P Lim; Kavita Garg; Mark Li; Lars D Engstrom; Laura Waters; J David Lawson; Peter Olson; Piro Lito; Sai-Hong I Ou; James G Christensen; Pasi A Jänne; Andrew J Aguirre
Journal:  N Engl J Med       Date:  2021-06-24       Impact factor: 91.245
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