| Literature DB >> 26235065 |
Anne Streeter1, Fiona M Menzies, David C Rubinsztein.
Abstract
The autophagosome-associated protein LC3-II is commonly used as a marker of autophagic activity within cells, but its levels are affected by both formation and degradation of autophagosomes. This can make the significance of altered LC3-II levels ambiguous. Here we describe the method of Bafilomycin A1 blotting, in which the degradation of autophagosomes is prevented in cultured cells, allowing the causes of altered LC3-II levels to be determined.Entities:
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Year: 2016 PMID： 26235065 DOI： 10.1007/978-1-4939-2627-5_8
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745