Literature DB >> 2623365

Determination of G + C content of DNA using high-performance liquid chromatography for the identification of staphylococci and micrococci.

M Peyret1, J Freney, H Meugnier, J Fleurette.   

Abstract

The guanine-plus-cytosine (G + C) content of different species of Staphylococcus and Micrococcus was determined by high-performance liquid chromatography. Purified bacterial DNA was hydrolysed by nuclease P1. The nucleotides were separated by chromatography and quantified by measurement of the optical density at 260 nm. The G + C content of staphylococci ranged from 31.5 to 37.9 moles %, and that of micrococci from 68.7 to 75.2. Most of our results were comparable to those obtained with the thermal method.

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Year:  1989        PMID: 2623365     DOI: 10.1016/0923-2508(89)90068-5

Source DB:  PubMed          Journal:  Res Microbiol        ISSN: 0923-2508            Impact factor:   3.992


  2 in total

1.  Production and Characterization of an Exopolysaccharide Excreted by a Deep-Sea Hydrothermal Vent Bacterium Isolated from the Polychaete Annelid Alvinella pompejana.

Authors:  P Vincent; P Pignet; F Talmont; L Bozzi; B Fournet; J Guezennec; C Jeanthon; D Prieur
Journal:  Appl Environ Microbiol       Date:  1994-11       Impact factor: 4.792

2.  Evaluation of API Coryne in comparison with conventional methods for identifying coryneform bacteria.

Authors:  J Freney; M T Duperron; C Courtier; W Hansen; F Allard; J M Boeufgras; D Monget; J Fleurette
Journal:  J Clin Microbiol       Date:  1991-01       Impact factor: 5.948

  2 in total

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