AIM: Our work aimed to designate the optimal DNA source for pharmacogenetic assays, such as the screening for HLA-B*57:01 allele. MATERIALS & METHODS: A saliva and four buccal swab samples were taken from 104 patients. All the samples were stored at different time and temperature conditions and then genotyped for the HLA-B*57:01 allele by SSP-PCR and classical/capillary electrophoresis. RESULTS: The genotyping analysis reported different performance rates depending on the storage conditions of the samples. Given our results, the buccal swab demonstrated to be more resistant and stable in time with respect to the saliva. CONCLUSION: Our investigation designates the buccal swab as the optimal DNA source for pharmacogenetic assays in terms of resistance, low infectivity, low-invasiveness and easy sampling, and safe transport in centralized medical centers providing specialized pharmacogenetic tests.
AIM: Our work aimed to designate the optimal DNA source for pharmacogenetic assays, such as the screening for HLA-B*57:01 allele. MATERIALS & METHODS: A saliva and four buccal swab samples were taken from 104 patients. All the samples were stored at different time and temperature conditions and then genotyped for the HLA-B*57:01 allele by SSP-PCR and classical/capillary electrophoresis. RESULTS: The genotyping analysis reported different performance rates depending on the storage conditions of the samples. Given our results, the buccal swab demonstrated to be more resistant and stable in time with respect to the saliva. CONCLUSION: Our investigation designates the buccal swab as the optimal DNA source for pharmacogenetic assays in terms of resistance, low infectivity, low-invasiveness and easy sampling, and safe transport in centralized medical centers providing specialized pharmacogenetic tests.
Authors: Thais Francini Garbieri; Daniel Thomas Brozoski; Thiago José Dionísio; Carlos Ferreira Santos; Lucimara Teixeira das Neves Journal: J Appl Oral Sci Date: 2017 Mar-Apr Impact factor: 2.698
Authors: Daniele Xavier Assad; Elisa Cançado Porto Mascarenhas; Ana Gabriela Costa Normando; Hélène Chardin; Gustavo Barcelos Barra; Riccardo Pratesi; Yanna Karla de Medeiros Nóbrega; Ana Carolina Acevedo; Eliete Neves Silva Guerra Journal: Mol Clin Oncol Date: 2020-06-04
Authors: G Aloe; C M De Sanctis; C Strafella; R Cascella; F Missiroli; M Cesareo; E Giardina; F Ricci Journal: Case Rep Ophthalmol Med Date: 2019-07-31