Qi-guang Chen1, Wei Zhou2, Tao Han3, Shu-qi Du1, Zhen-hua Li1, Zhe Zhang1, Guang-yi Shan4, Chui-ze Kong5. 1. Department of Urology, The First Affiliated Hospital of China Medical University, Nanjing Street 155#, Shenyang, 110001, Liaoning, China. 2. Department of Diagnostic Radiology, General Hospital of Shenyang Military Region, Shenyang, China. 3. Department of Oncology, General Hospital of Shenyang Military Region, Shenyang, China. 4. Department of Urology, LiaoNing Cancer Hospital and Institute, Shenyang, China. 5. Department of Urology, The First Affiliated Hospital of China Medical University, Nanjing Street 155#, Shenyang, 110001, Liaoning, China. kongchuize408@163.com.
Abstract
INTRODUCTION: The roles of dysregulated microRNAs in prostate cancer metastasis are still unknown. In this study, we found that the expression of miR-345 was significantly downregulated in prostate cancer and clinical prostate cancer tissues. MATERIALS, METHODS AND RESULTS: Overexpression of miR-345 in prostate cancer cells suppressed proliferation, migration and invasion. Using nude mice model, we revealed that miR-345 inhibits the growth of prostate cancer cells in vivo and in vitro. Furthermore, we identified and validated Smad1 as a direct target of miR-345. Ectopic expression of Smad1 without its 3'-UTR rescued miR-345-induced cell migration and invasion inhibition. CONCLUSION: Taken together, our data suggest that miR-345 exerts a suppressive effect on prostate cancer proliferation, invasion and migration through downregulation of Smad1.
INTRODUCTION: The roles of dysregulated microRNAs in prostate cancer metastasis are still unknown. In this study, we found that the expression of miR-345 was significantly downregulated in prostate cancer and clinical prostate cancer tissues. MATERIALS, METHODS AND RESULTS: Overexpression of miR-345 in prostate cancer cells suppressed proliferation, migration and invasion. Using nude mice model, we revealed that miR-345 inhibits the growth of prostate cancer cells in vivo and in vitro. Furthermore, we identified and validated Smad1 as a direct target of miR-345. Ectopic expression of Smad1 without its 3'-UTR rescued miR-345-induced cell migration and invasion inhibition. CONCLUSION: Taken together, our data suggest that miR-345 exerts a suppressive effect on prostate cancer proliferation, invasion and migration through downregulation of Smad1.
Authors: Jason C Gonzales; Louis M Fink; Oscar B Goodman; James T Symanowski; Nicholas J Vogelzang; David C Ward Journal: Clin Genitourin Cancer Date: 2011-07-01 Impact factor: 2.872
Authors: Yubin Hao; Xinbin Gu; Yuan Zhao; Stephen Greene; Wei Sha; Duane T Smoot; Joseph Califano; T-C Wu; Xiaowu Pang Journal: Cancer Prev Res (Phila) Date: 2011-03-23
Authors: Kirsten L Greene; Maxwell V Meng; Eric P Elkin; Matthew R Cooperberg; David J Pasta; Michael W Kattan; Katrine Wallace; Peter R Carroll Journal: J Urol Date: 2004-06 Impact factor: 7.450
Authors: E Danese; A M Minicozzi; M Benati; E Paviati; G Lima-Oliveira; M Gusella; F Pasini; G L Salvagno; M Montagnana; G Lippi Journal: Sci Rep Date: 2017-08-21 Impact factor: 4.379