| Literature DB >> 26227036 |
Clément Dégut1, Alexandre Monod, Franck Brachet, Thibaut Crépin, Carine Tisné.
Abstract
tRNAs occupy a central role in the cellular life, and they are involved in a broad range of biological processes that relies on their interaction with proteins and RNA. Crystallization and structure resolution of tRNA or/and tRNA/partner complexes can yield in valuable information on structural organizations of key elements of cellular machinery. However, crystallization of RNA, is often challenging. Here we review two methods to produce and purify tRNA in quantity and quality to perform X-ray studies.Entities:
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Year: 2016 PMID： 26227036 DOI： 10.1007/978-1-4939-2763-0_4
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745