Literature DB >> 26226621

Heavy water: a simple solution to increasing the brightness of fluorescent proteins in super-resolution imaging.

Wei Qiang Ong1, Y Rose Citron, Joerg Schnitzbauer, Daichi Kamiyama, Bo Huang.   

Abstract

Photoactivatable fluorescent proteins (PA-FPs) are widely used in live single-molecule super-resolution imaging but emit substantially fewer photons than organic dyes do. Herein, we show that in heavy water (D2O) instead of H2O, common PA-FPs emit 26-54% more photons, effectively improving the localization precision in super-resolution imaging.

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Year:  2015        PMID： 26226621      PMCID： PMC4544551          DOI： 10.1039/c5cc04575d

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  31 in total

1.  A photoactivatable GFP for selective photolabeling of proteins and cells.

Authors:  George H Patterson; Jennifer Lippincott-Schwartz
Journal:  Science       Date:  2002-09-13       Impact factor: 47.728

2.  Rational design of true monomeric and bright photoactivatable fluorescent proteins.

Authors:  Mingshu Zhang; Hao Chang; Yongdeng Zhang; Junwei Yu; Lijie Wu; Wei Ji; Juanjuan Chen; Bei Liu; Jingze Lu; Yingfang Liu; Junlong Zhang; Pingyong Xu; Tao Xu
Journal:  Nat Methods       Date:  2012-05-13       Impact factor: 28.547

3.  Improved super-resolution microscopy with oxazine fluorophores in heavy water.

Authors:  Steven F Lee; Quentin Vérolet; Alexandre Fürstenberg
Journal:  Angew Chem Int Ed Engl       Date:  2013-07-04       Impact factor: 15.336

4.  Global analysis of the thermal and chemical denaturation of the N-terminal domain of the ribosomal protein L9 in H2O and D2O. Determination of the thermodynamic parameters, deltaH(o), deltaS(o), and deltaC(o)p and evaluation of solvent isotope effects.

Authors:  B Kuhlman; D P Raleigh
Journal:  Protein Sci       Date:  1998-11       Impact factor: 6.725

5.  Characterization and development of photoactivatable fluorescent proteins for single-molecule-based superresolution imaging.

Authors:  Siyuan Wang; Jeffrey R Moffitt; Graham T Dempsey; X Sunney Xie; Xiaowei Zhuang
Journal:  Proc Natl Acad Sci U S A       Date:  2014-05-27       Impact factor: 11.205

6.  Solvent isotope effect and protein stability.

Authors:  G I Makhatadze; G M Clore; A M Gronenborn
Journal:  Nat Struct Biol       Date:  1995-10

7.  Cold denaturation and 2H2O stabilization of a staphylococcal nuclease mutant.

Authors:  L C Antonino; R A Kautz; T Nakano; R O Fox; A L Fink
Journal:  Proc Natl Acad Sci U S A       Date:  1991-09-01       Impact factor: 11.205

8.  Photoactivatable mCherry for high-resolution two-color fluorescence microscopy.

Authors:  Fedor V Subach; George H Patterson; Suliana Manley; Jennifer M Gillette; Jennifer Lippincott-Schwartz; Vladislav V Verkhusha
Journal:  Nat Methods       Date:  2009-01-25       Impact factor: 28.547

9.  Increasing the brightness of cyanine fluorophores for single-molecule and superresolution imaging.

Authors:  Kathrin Klehs; Christoph Spahn; Ulrike Endesfelder; Steven F Lee; Alexandre Fürstenberg; Mike Heilemann
Journal:  Chemphyschem       Date:  2013-12-02       Impact factor: 3.102

10.  A bright and photostable photoconvertible fluorescent protein.

Authors:  Sean A McKinney; Christopher S Murphy; Kristin L Hazelwood; Michael W Davidson; Loren L Looger
Journal:  Nat Methods       Date:  2009-01-25       Impact factor: 28.547
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  6 in total

Review 1.  Switchable Fluorophores for Single-Molecule Localization Microscopy.

Authors:  Honglin Li; Joshua C Vaughan
Journal:  Chem Rev       Date:  2018-09-17       Impact factor: 60.622

2.  Ultra-High Resolution 3D Imaging of Whole Cells.

Authors:  Fang Huang; George Sirinakis; Edward S Allgeyer; Lena K Schroeder; Whitney C Duim; Emil B Kromann; Thomy Phan; Felix E Rivera-Molina; Jordan R Myers; Irnov Irnov; Mark Lessard; Yongdeng Zhang; Mary Ann Handel; Christine Jacobs-Wagner; C Patrick Lusk; James E Rothman; Derek Toomre; Martin J Booth; Joerg Bewersdorf
Journal:  Cell       Date:  2016-07-07       Impact factor: 41.582

3.  Molecular coordination of Staphylococcus aureus cell division.

Authors:  Victoria A Lund; Katarzyna Wacnik; Robert D Turner; Bryony E Cotterell; Christa G Walther; Samuel J Fenn; Fabian Grein; Adam Jm Wollman; Mark C Leake; Nicolas Olivier; Ashley Cadby; Stéphane Mesnage; Simon Jones; Simon J Foster
Journal:  Elife       Date:  2018-02-21       Impact factor: 8.140

4.  Systematic Nanoscale Analysis of Endocytosis Links Efficient Vesicle Formation to Patterned Actin Nucleation.

Authors:  Markus Mund; Johannes Albertus van der Beek; Joran Deschamps; Serge Dmitrieff; Philipp Hoess; Jooske Louise Monster; Andrea Picco; François Nédélec; Marko Kaksonen; Jonas Ries
Journal:  Cell       Date:  2018-07-26       Impact factor: 41.582

Review 5.  Bleaching-Resistant Super-Resolution Fluorescence Microscopy.

Authors:  Jiwoong Kwon; Mohamed Saleh Elgawish; Sang-Hee Shim
Journal:  Adv Sci (Weinh)       Date:  2022-01-27       Impact factor: 16.806

6.  FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells.

Authors:  Srinjan Basu; Lisa-Maria Needham; David Lando; Edward J R Taylor; Kai J Wohlfahrt; Devina Shah; Wayne Boucher; Yi Lei Tan; Lawrence E Bates; Olga Tkachenko; Julie Cramard; B Christoffer Lagerholm; Christian Eggeling; Brian Hendrich; Dave Klenerman; Steven F Lee; Ernest D Laue
Journal:  Nat Commun       Date:  2018-06-28       Impact factor: 14.919
  6 in total

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