Kaushik Patnaik1, A R Pradeep1, Kanika Nagpal1, Shruti Karvekar1, Priyanka Singh2, Arjun Raju3. 1. Department of Periodontics, Government Dental College and Research Institute, Bangalore, Karnataka, India. 2. Department of Ophthalmology, Grant Medical College and J. J. Group of Hospitals, Mumbai, India. 3. Department of Radio-Diagnosis, Uttarakhand Government Medical College, Haldwani, Nainital, Uttarakhand, India.
Abstract
AIM: The aim of the present study was to evaluate the levels and correlation of human chemerin in gingival crevicular fluid (GCF) and tear fluid in chronic periodontitis (CP) subjects with and without type-2 diabetes mellitus (DM). MATERIALS AND METHODS: A total of 40 subjects were divided into three groups: group 1 had 10 periodontally healthy subjects; group 2 consisted of 15 CP subjects and group 3 had 15 type-2 DM subjects with CP. The GCF and tear fluid levels of human chemerin were quantified using enzyme linked immunosorbent assay (ELISA). Systemic parameters such as body mass index, glycated hemoglobin levels and fasting plasma glucose levels were evaluated. The clinical outcomes evaluated were gingival index (GI), probing depth (PD), and clinical attachment level (CAL), and the correlations of the two inflammatory mediators with clinical parameters were evaluated. RESULTS: Human chemerin levels increased from group 1 to group 2 to group 3. The GCF and tear-fluid values of the inflammatory mediators correlated positively with each other and with the evaluated periodontal parameters (P < 0.05). CONCLUSION: Human chemerin can be considered as possible GCF and tear-fluid markers of inflammatory activity in CP and DM.
AIM: The aim of the present study was to evaluate the levels and correlation of humanchemerin in gingival crevicular fluid (GCF) and tear fluid in chronic periodontitis (CP) subjects with and without type-2 diabetes mellitus (DM). MATERIALS AND METHODS: A total of 40 subjects were divided into three groups: group 1 had 10 periodontally healthy subjects; group 2 consisted of 15 CP subjects and group 3 had 15 type-2 DM subjects with CP. The GCF and tear fluid levels of humanchemerin were quantified using enzyme linked immunosorbent assay (ELISA). Systemic parameters such as body mass index, glycated hemoglobin levels and fasting plasma glucose levels were evaluated. The clinical outcomes evaluated were gingival index (GI), probing depth (PD), and clinical attachment level (CAL), and the correlations of the two inflammatory mediators with clinical parameters were evaluated. RESULTS:Humanchemerin levels increased from group 1 to group 2 to group 3. The GCF and tear-fluid values of the inflammatory mediators correlated positively with each other and with the evaluated periodontal parameters (P < 0.05). CONCLUSION:Humanchemerin can be considered as possible GCF and tear-fluid markers of inflammatory activity in CP and DM.
Authors: Urszula Godlewska; Piotr Brzoza; Aneta Sroka; Pawel Majewski; Holger Jentsch; Martin Eckert; Sigrun Eick; Jan Potempa; Brian A Zabel; Joanna Cichy Journal: Front Immunol Date: 2017-03-29 Impact factor: 7.561