U Can1, M Buyukinan2, F H Yerlikaya3. 1. Department of Biochemistry, Konya Education and Research Hospital, Konya, Turkey. 2. Department of Pediatric Endocrinology, Konya Education and Research Hospital, Konya, Turkey. 3. Department of Biochemistry, Meram Faculty of Medicine, University of Necmettin Erbakan, Konya, Turkey.
Abstract
BACKGROUND: Childhood obesity is an increasing health challenge related to increased risk of chronic diseases. microRNAs (miRNAs) are noncoding short RNA molecules regulating multiple biological processes linked to obesity. OBJECTIVES: We aimed at evaluating the association between circulating miRNA levels and lipid metabolism in obese and non-obese children and adolescents. METHODS: By constituting study group, 45 obese children and adolescents were recruited. To perform comparisons with study group, 41 lean controls were matched for age and sex. Using real-time quantitative PCR analysis, circulating miRNAs were evaluated in both groups. RESULTS: Circulating miR-335 (P < 0.001), miR-143 (P = 0.001) and miR-758 (P = 0.006) in obese children were significantly lower than those of controls. However, circulating miR-27 (P = 0.032), miR-378 (P < 0.001) and miR-370 (P = 0.045) in obese children were significantly higher, compared with those of controls. In addition, circulating miR-33 in obese children was higher than those of controls, but no significant difference was present (P = 0.687). CONCLUSION: Our findings showed that a significant association is present between circulating miR-370, miR-33, miR-378, miR-27, miR-335, miR-143 and miR-758 values, and childhood obesity. Low levels of miR-335, miR-143 and miR-758, and high levels of miR-27, miR-378, miR-33 and miR-370 may have been responsible for elevated triglycerides and low-density lipoprotein (LDL-C) levels, and low level of high-density lipoprotein (HDL-C) in obese subjects. Therefore, miRNAs may be a good novel biomarker for childhood obesity.
BACKGROUND: Childhood obesity is an increasing health challenge related to increased risk of chronic diseases. microRNAs (miRNAs) are noncoding short RNA molecules regulating multiple biological processes linked to obesity. OBJECTIVES: We aimed at evaluating the association between circulating miRNA levels and lipid metabolism in obese and non-obesechildren and adolescents. METHODS: By constituting study group, 45 obesechildren and adolescents were recruited. To perform comparisons with study group, 41 lean controls were matched for age and sex. Using real-time quantitative PCR analysis, circulating miRNAs were evaluated in both groups. RESULTS: Circulating miR-335 (P < 0.001), miR-143 (P = 0.001) and miR-758 (P = 0.006) in obesechildren were significantly lower than those of controls. However, circulating miR-27 (P = 0.032), miR-378 (P < 0.001) and miR-370 (P = 0.045) in obesechildren were significantly higher, compared with those of controls. In addition, circulating miR-33 in obesechildren was higher than those of controls, but no significant difference was present (P = 0.687). CONCLUSION: Our findings showed that a significant association is present between circulating miR-370, miR-33, miR-378, miR-27, miR-335, miR-143 and miR-758 values, and childhood obesity. Low levels of miR-335, miR-143 and miR-758, and high levels of miR-27, miR-378, miR-33 and miR-370 may have been responsible for elevated triglycerides and low-density lipoprotein (LDL-C) levels, and low level of high-density lipoprotein (HDL-C) in obese subjects. Therefore, miRNAs may be a good novel biomarker for childhood obesity.
Authors: Marcelo A Mori; Raissa G Ludwig; Ruben Garcia-Martin; Bruna B Brandão; C Ronald Kahn Journal: Cell Metab Date: 2019-08-22 Impact factor: 27.287
Authors: Simona Riccio; Rosa Melone; Caterina Vitulano; Pierfrancesco Guida; Ivan Maddaluno; Stefano Guarino; Pierluigi Marzuillo; Emanuele Miraglia Del Giudice; Anna Di Sessa Journal: World J Clin Pediatr Date: 2022-03-23
Authors: Giuseppe Iacomino; Paola Russo; Pasquale Marena; Fabio Lauria; Antonella Venezia; Wolfgang Ahrens; Stefaan De Henauw; Pasquale De Luca; Ronja Foraita; Kathrin Günther; Lauren Lissner; Dénes Molnár; Luis A Moreno; Michael Tornaritis; Toomas Veidebaum; Alfonso Siani Journal: Genes Nutr Date: 2019-01-09 Impact factor: 5.523