Elva I Cortés-Gutiérrez1, Martha I Dávila-Rodríguez2, José Luis Fernández3,4, Carmen López-Fernández5, Anel R Aragón-Tovar6, Luis C Urbina-Bernal6, Jaime Gosálvez5. 1. Department of Genetics, Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, IMSS, 64720, Monterrey, Nuevo León, Mexico. elvacortes@cibinmty.net. 2. Department of Genetics, Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, IMSS, 64720, Monterrey, Nuevo León, Mexico. 3. Genetics Unit, INIBIC, Complejo Hospitalario Universitario A Coruña, As Xubias 84, 15006, La Coruña, Spain. 4. Laboratorio de Genética Molecular y Radiobiología, Centro Oncológico de Galicia, Doctor Camilo Veiras 1, 15009, La Coruña, Spain. 5. Unit of Genetics, Department of Biology, Universidad Autónoma de Madrid, 20849, Madrid, Spain. 6. Department of Urology, Unidad Medica de Alta Especialidad No. 25, IMSS, Monterrey, Mexico.
Abstract
PURPOSE: Evaluation of DNA integrity is an important test, possessing greater diagnostic and prognostic significance for couples requiring assisted reproduction. In this study, we evaluate the levels of DNA damage in infertile patients with varicocele with respect to fertile males by the sperm chromatin dispersion (SCD) test. The presence of DNA breaks in spermatozoa was confirmed by DNA breakage detection-fluorescence in situ hybridization (DBD-FISH). METHODS: In this study, the frequency of sperm cells with fragmented DNA was studied in a group of 20 infertile patients with varicocele and compared with 20 fertile males. The spermatozoa were processed to classify different levels of DNA fragmentation using the Halosperm(®) kit, an improved SCD test, and DBD-FISH. RESULTS: Patients with varicocele showed 25.54 ± 28.17 % of spermatozoa with fragmented DNA, significantly higher than those of the group of fertile subjects (11.54 ± 3.88 %). The proportion of degraded cells in total sperm cells with fragmented DNA was sixfold higher in the case of patients with varicocele. The presence of DNA breaks in spermatozoa was confirmed by DBD-FISH. 5-bp Classical satellite-2 regions showed greater sensitivity to damage or "breakage" than alphoid satellite regions. CONCLUSIONS: Our finding preliminary demonstrated an increase of DNA fragmentation associated to severe sperm damage, in infertile patients with varicocele with respect to fertile males. 5-bp Classical satellite-2 regions showed greater sensitivity to damage or "breakage" than alphoid satellite regions.
PURPOSE: Evaluation of DNA integrity is an important test, possessing greater diagnostic and prognostic significance for couples requiring assisted reproduction. In this study, we evaluate the levels of DNA damage in infertilepatients with varicocele with respect to fertile males by the sperm chromatin dispersion (SCD) test. The presence of DNA breaks in spermatozoa was confirmed by DNA breakage detection-fluorescence in situ hybridization (DBD-FISH). METHODS: In this study, the frequency of sperm cells with fragmented DNA was studied in a group of 20 infertilepatients with varicocele and compared with 20 fertile males. The spermatozoa were processed to classify different levels of DNA fragmentation using the Halosperm(®) kit, an improved SCD test, and DBD-FISH. RESULTS:Patients with varicocele showed 25.54 ± 28.17 % of spermatozoa with fragmented DNA, significantly higher than those of the group of fertile subjects (11.54 ± 3.88 %). The proportion of degraded cells in total sperm cells with fragmented DNA was sixfold higher in the case of patients with varicocele. The presence of DNA breaks in spermatozoa was confirmed by DBD-FISH. 5-bp Classical satellite-2 regions showed greater sensitivity to damage or "breakage" than alphoid satellite regions. CONCLUSIONS: Our finding preliminary demonstrated an increase of DNA fragmentation associated to severe sperm damage, in infertilepatients with varicocele with respect to fertile males. 5-bp Classical satellite-2 regions showed greater sensitivity to damage or "breakage" than alphoid satellite regions.
Entities:
Keywords:
DNA breakage detection-fluorescence in situ hybridization, DBD-FISH; DNA fragmentation; Human sperm; Sperm chromatin dispersion; Varicocele
Authors: Andrea Sansone; Danilo Alunni Fegatelli; Carlotta Pozza; Giorgio Fattorini; Rosa Lauretta; Marianna Minnetti; Francesco Romanelli; Pierleone Lucatelli; Mario Corona; Mario Bezzi; Francesco Lombardo; Andrea Lenzi; Daniele Gianfrilli Journal: Asian J Androl Date: 2019 Jul-Aug Impact factor: 3.285
Authors: Monika Fraczek; Angelika Lewandowska; Marta Budzinska; Marzena Kamieniczna; Lukasz Wojnar; Kamil Gill; Malgorzata Piasecka; Michal Kups; Anna Havrylyuk; Valentina Chopyak; Jozef Nakonechnyy; Andrij Nakonechnyy; Maciej Kurpisz Journal: Int J Environ Res Public Health Date: 2022-02-25 Impact factor: 3.390