OBJECTIVE: This study aims to observe the contraction role of RELMα in rat aortic smooth muscle cells and explore its mechanism. METHODS: Rat aortas smooth muscle cells were cultivated using tissue explants method. They were divided into 5 groups. A: Control group; B: 1×10(-7) mol/L ANGII group; C: 1×10(-8) mol/L RELMα group; D: 2×10(-8) mol/L RELMα group; E: 4×10(-8) mol/L RELMα group. The thoracic aortic tension signal of rats was recorded by Powerlab system. The expression levels of CaM and MLCK were detected by western blotting and RT-PCR methods. RESULTS: Tension changes of rat thoracic aorta vascular ring in group C, D and E (72±2.98%, 76.65±2.73%, 85.07±3.06% respectively) were higher than that of group A and B (6.35±0.75%, 61.47±4.47%) with dose-dependent (P<0.01). The expression levels of CaM and MLCK in group C were higher than that of group A and B while were lower than that of group D and E (P<0.05). The expression levels of CaM and MLCK in group E were the highest among the groups (P<0.05). CONCLUSIONS: RELMα can cause contraction of rat aortic smooth muscle cells, its mechanism may be via Ca(2+)-CaM-MLCK pathway.
OBJECTIVE: This study aims to observe the contraction role of RELMα in rat aortic smooth muscle cells and explore its mechanism. METHODS:Rat aortas smooth muscle cells were cultivated using tissue explants method. They were divided into 5 groups. A: Control group; B: 1×10(-7) mol/L ANGII group; C: 1×10(-8) mol/L RELMα group; D: 2×10(-8) mol/L RELMα group; E: 4×10(-8) mol/L RELMα group. The thoracic aortic tension signal of rats was recorded by Powerlab system. The expression levels of CaM and MLCK were detected by western blotting and RT-PCR methods. RESULTS: Tension changes of rat thoracic aorta vascular ring in group C, D and E (72±2.98%, 76.65±2.73%, 85.07±3.06% respectively) were higher than that of group A and B (6.35±0.75%, 61.47±4.47%) with dose-dependent (P<0.01). The expression levels of CaM and MLCK in group C were higher than that of group A and B while were lower than that of group D and E (P<0.05). The expression levels of CaM and MLCK in group E were the highest among the groups (P<0.05). CONCLUSIONS: RELMα can cause contraction of rat aortic smooth muscle cells, its mechanism may be via Ca(2+)-CaM-MLCK pathway.
Entities:
Keywords:
Aortas; RELMα; cell contraction; smooth muscle cells; vascular ring
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