Sarah A Engelberth1, Nadine Hempel2, Magnus Bergkvist1. 1. †Nanobioscience Constellation, College of Nanoscale Science and Engineering, SUNY Polytechnic Institute, Albany, New York 12203, United States. 2. ‡Department of Pharmacology, Penn State College of Medicine, Hershey, Pennsylvania 17033, United States.
Abstract
Nanostructured starches are naturally derived nanomaterials that can be chemically modified to allow for the introduction of functional groups, enhancing their potential for drug delivery and other biotechnology applications. In this proof of concept study, we investigate chemically modified, enzymatically synthesized glycogen (ESG) nanodendrites as a biodegradable, biocompatible, siRNA delivery system. Commercially available ESG was modified using glycidyltrimethylammonium chloride (GTMA), introducing quaternary ammonium groups via an epoxide ring opening reaction. This cationic ESG (cESG) electrostatically bound siRNA and successfully knocked down protein expression in an in vitro ovarian clear cell carcinoma model. The construct exhibited sustained siRNA delivery for up to 6 days while exhibiting less toxicity than a common liposome-based siRNA delivery reagent, Lipofectamine RNAiMAX. These promising results set the stage for the use of dendritic starch as a cost-effective, easily modifiable nanoscale delivery system for a diverse range of cargo including nucleic acids and therapeutic compounds.
Nanostructured n class="Chemical">starches are naturally derived nanomaterials that can be chemically modified to allow for the introduction of functional groups, enhancing their potential for drug delivery and other biotechnology applications. In this proof of concept study, we investigate chemically modified, enzymatically synthesized glycogen (ESG) nanodendrites as a biodegradable, biocompatible, siRNA delivery system. Commercially available ESG was modified using glycidyltrimethylammonium chloride (GTMA), introducing quaternary ammonium groups via an epoxidering opening reaction. This cationic ESG (cESG) electrostatically bound siRNA and successfully knocked down protein expression in an in vitro ovarian clear cell carcinoma model. The construct exhibited sustained siRNA delivery for up to 6 days while exhibiting less toxicity than a common liposome-based siRNA delivery reagent, Lipofectamine RNAiMAX. These promising results set the stage for the use of dendritic starch as a cost-effective, easily modifiable nanoscale delivery system for a diverse range of cargo including nucleic acids and therapeutic compounds.
Authors: D Dauvillée; C Colleoni; G Mouille; A Buléon; D J Gallant; B Bouchet; M K Morell; C d'Hulst; A M Myers; S G Ball Journal: Plant Physiol Date: 2001-04 Impact factor: 8.340
Authors: Rubén Gómez-Sánchez; Elisa Pizarro-Estrella; Sokhna M S Yakhine-Diop; Mario Rodríguez-Arribas; José M Bravo-San Pedro; José M Fuentes; Rosa A González-Polo Journal: Anal Biochem Date: 2015-03-04 Impact factor: 3.365
Authors: Rachel A Burton; Helen Jenner; Luke Carrangis; Brendan Fahy; Geoffrey B Fincher; Chris Hylton; David A Laurie; Mary Parker; Darren Waite; Sonja van Wegen; Tamara Verhoeven; Kay Denyer Journal: Plant J Date: 2002-07 Impact factor: 6.417
Authors: Usawadee Dier; Dong-Hui Shin; L P Madhubhani P Hemachandra; Larissa M Uusitalo; Nadine Hempel Journal: PLoS One Date: 2014-05-23 Impact factor: 3.240
Authors: Elena Favaro; Karim Bensaad; Mei G Chong; Daniel A Tennant; David J P Ferguson; Cameron Snell; Graham Steers; Helen Turley; Ji-Liang Li; Ulrich L Günther; Francesca M Buffa; Alan McIntyre; Adrian L Harris Journal: Cell Metab Date: 2012-11-21 Impact factor: 27.287