Literature DB >> 26215382

Initial clinical experience with BRAF(V600E) mutation analysis of core-needle biopsy specimens from thyroid nodules.

Sang Hyun Choi1, Jung Hwan Baek1, Jeong Hyun Lee1, Young Jun Choi1, Eun Ju Ha1,2, Dong Eun Song3, Jae Kyun Kim4, Ki-Wook Chung5, Tae Yong Kim6, Won Bae Kim6, Young Kee Shong6.   

Abstract

OBJECTIVE: The accurate diagnosis of thyroid nodules is important for making management decisions. The purpose of this study was to evaluate the feasibility of core-needle biopsy with BRAF(V600E) mutation analysis (CNB + BRAF(V600E) ) and to compare the clinical usefulness of CNB + BRAF(V600E) and fine-needle aspiration with BRAF(V600E) mutation analysis (FNA + BRAF(V600E) ) in the diagnosis of thyroid malignancy. DESIGN, PATIENTS AND MEASUREMENTS: The results of BRAF(V600E) mutation analyses of 820 nodules from 820 patients (153 men, 667 women; mean age, 51·1 years), who underwent CNB + BRAF(V600E) (n = 256) or FNA + BRAF(V600E) (n = 564) between January 2011 and March 2012 were retrospectively evaluated. The feasibility of CNB + BRAF(V600E) was assessed by comparing its rate of detection of BRAF(V600E) mutations and positive predictive value with those of FNA + BRAF(V600E) . The clinical usefulness of CNB + BRAF(V600E) was determined by comparing rates of inconclusive results, the additional value of BRAF(V600E) mutation analysis, diagnostic surgery and diagnostic performance with those of FNA + BRAF(V600E) .
RESULTS: CNB + BRAF(V600E) and FNA + BRAF(V600E) had similar rates of BRAF(V600E) mutation detection (66·3% vs 64·4%, P = 0·883) and positive predictive value (100·0% vs 96·6%, P = 0·135). CNB + BRAF(V600E) had a significantly higher diagnostic accuracy rate (95·7% vs 85·9%, P < 0·001), and significantly lower rates of inconclusive results (8·2% vs 51·8%, P < 0·001), and diagnostic surgery (8·9% vs 22·3%, P = 0·006), than FNA + BRAF(V600E) .
CONCLUSIONS: The greater diagnostic performance of CNB + BRAF(V600E) and its lower rate of inconclusive results than FNA + BRAF(V600E) suggest that CNB + BRAF(V600E) can reduce rates of preventable diagnostic surgery.
© 2015 John Wiley & Sons Ltd.

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Year:  2015        PMID: 26215382     DOI: 10.1111/cen.12866

Source DB:  PubMed          Journal:  Clin Endocrinol (Oxf)        ISSN: 0300-0664            Impact factor:   3.478


  2 in total

1.  Comparison of Immunohistochemistry and Direct Sanger Sequencing for Detection of the BRAF(V600E) Mutation in Thyroid Neoplasm.

Authors:  Hye Seon Oh; Hyemi Kwon; Suyeon Park; Mijin Kim; Min Ji Jeon; Tae Yong Kim; Young Kee Shong; Won Bae Kim; Jene Choi; Won Gu Kim; Dong Eun Song
Journal:  Endocrinol Metab (Seoul)       Date:  2018-01-30

2.  Application of biomarkers in the diagnosis of uncertain samples of core needle biopsy of thyroid nodules.

Authors:  Yan Xiong; Xin Li; Li Liang; Dong Li; Limin Yan; Xueying Li; Jiting Di; Ting Li
Journal:  Virchows Arch       Date:  2021-07-26       Impact factor: 4.064

  2 in total

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