Literature DB >> 26215183

Validation of QTL for resistance to Aphanomyces euteiches in different pea genetic backgrounds using near-isogenic lines.

C Lavaud1, A Lesné1,2, C Piriou1,3, G Le Roy1,3, G Boutet1,3, A Moussart3,4, C Poncet5, R Delourme1, A Baranger1,3, M-L Pilet-Nayel6,7.   

Abstract

KEY MESSAGE: Marker-assisted backcrossing was used to generate pea NILs carrying individual or combined resistance alleles at main Aphanomyces resistance QTL. The effects of several QTL were successfully validated depending on genetic backgrounds. Quantitative trait loci (QTL) validation is an important and often overlooked step before subsequent research in QTL cloning or marker-assisted breeding for disease resistance in plants. Validation of QTL controlling partial resistance to Aphanomyces root rot, one of the most damaging diseases of pea worldwide, is of major interest for the future development of resistant varieties. The aim of this study was to validate, in different genetic backgrounds, the effects of various resistance alleles at seven main resistance QTL recently identified. Five backcross-assisted selection programs were developed. In each, resistance alleles at one to three of the seven main Aphanomyces resistance QTL were transferred into three genetic backgrounds, including two agronomically important spring (Eden) and winter (Isard) pea cultivars. The subsequent near-isogenic lines (NILs) were evaluated for resistance to two reference strains of the main A. euteiches pathotypes under controlled conditions. The NILs carrying resistance alleles at the major-effect QTL Ae-Ps4.5 and Ae-Ps7.6, either individually or in combination with resistance alleles at other QTL, showed significantly reduced disease severity compared to NILs without resistance alleles. Resistance alleles at some minor-effect QTL, especially Ae-Ps2.2 and Ae-Ps5.1, were also validated for their individual or combined effects on resistance. QTL × genetic background interactions were observed, mainly for QTL Ae-Ps7.6, the effect of which increased in the winter cultivar Isard. The pea NILs are a novel and valuable resource for further understanding the mechanisms underlying QTL and their integration in breeding programs.

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Year:  2015        PMID： 26215183     DOI： 10.1007/s00122-015-2583-0

Source DB:  PubMed          Journal:  Theor Appl Genet        ISSN： 0040-5752            Impact factor:   5.699


  29 in total

1.  QTL x environment interactions in rice. I. heading date and plant height.

Authors:  Z K Li; S B Yu; H R Lafitte; N Huang; B Courtois; S Hittalmani; C H M Vijayakumar; G F Liu; G C Wang; H E Shashidhar; J Y Zhuang; K L Zheng; V P Singh; J S Sidhu; S Srivantaneeyakul; G S Khush
Journal:  Theor Appl Genet       Date:  2003-09-05       Impact factor: 5.699

2.  Power and false-positive rate in QTL detection with near-isogenic line libraries.

Authors:  K C Falke; M Frisch
Journal:  Heredity (Edinb)       Date:  2010-08-04       Impact factor: 3.821

3.  Development of wheat near-isogenic lines for powdery mildew resistance.

Authors:  Ronghua Zhou; Zhendong Zhu; Xiuying Kong; Naxin Huo; Qingzhen Tian; Pei Li; Cuiyun Jin; Yuchen Dong; Jizeng Jia
Journal:  Theor Appl Genet       Date:  2005-01-26       Impact factor: 5.699

4.  Microsatellite marker polymorphism and mapping in pea (Pisum sativum L.).

Authors:  K Loridon; K McPhee; J Morin; P Dubreuil; M L Pilet-Nayel; G Aubert; C Rameau; A Baranger; C Coyne; I Lejeune-Hènaut; J Burstin
Journal:  Theor Appl Genet       Date:  2005-10-11       Impact factor: 5.699

Review 5.  Selection in backcross programmes.

Authors:  Frédéric Hospital
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2005-07-29       Impact factor: 6.237

6.  Tagging genes for blast resistance in rice via linkage to RFLP markers.

Authors:  Z H Yu; D J Mackill; J M Bonman; S D Tanksley
Journal:  Theor Appl Genet       Date:  1991-04       Impact factor: 5.699

7.  Evaluation of near-isogenic lines for drought resistance QTL and fine mapping of a locus affecting flag leaf width, spikelet number, and root volume in rice.

Authors:  Xipeng Ding; Xiaokai Li; Lizhong Xiong
Journal:  Theor Appl Genet       Date:  2011-06-17       Impact factor: 5.699

8.  Marker-assisted introgression of quantitative trait loci.

Authors:  F Hospital; A Charcosset
Journal:  Genetics       Date:  1997-11       Impact factor: 4.562

9.  Fine-mapping of a major QTL controlling angular leaf spot resistance in common bean (Phaseolus vulgaris L.).

Authors:  Beat Keller; Chloe Manzanares; Carlos Jara; Juan David Lobaton; Bruno Studer; Bodo Raatz
Journal:  Theor Appl Genet       Date:  2015-03-05       Impact factor: 5.699

10.  QTL meta-analysis provides a comprehensive view of loci controlling partial resistance to Aphanomyces euteiches in four sources of resistance in pea.

Authors:  Céline Hamon; Clarice J Coyne; Rebecca J McGee; Angélique Lesné; Robert Esnault; Pierre Mangin; Marie Hervé; Isabelle Le Goff; Gwenaëlle Deniot; Martine Roux-Duparque; Gérard Morin; Kevin E McPhee; Régine Delourme; Alain Baranger; Marie-Laure Pilet-Nayel
Journal:  BMC Plant Biol       Date:  2013-03-16       Impact factor: 4.215
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  19 in total

Review 1.  Omics resources and omics-enabled approaches for achieving high productivity and improved quality in pea (Pisum sativum L.).

Authors:  Arun K Pandey; Diego Rubiales; Yonggang Wang; Pingping Fang; Ting Sun; Na Liu; Pei Xu
Journal:  Theor Appl Genet       Date:  2021-01-12       Impact factor: 5.699

2.  A major-effect genetic locus, ApRVII, controlling resistance against both adapted and non-adapted aphid biotypes in pea.

Authors:  Marie-Laure Pilet-Nayel; Jean-Christophe Simon; Akiko Sugio; Rémi Ollivier; Isabelle Glory; Romuald Cloteau; Jean-François Le Gallic; Gaëtan Denis; Stéphanie Morlière; Henri Miteul; Jean-Philippe Rivière; Angélique Lesné; Anthony Klein; Grégoire Aubert; Jonathan Kreplak; Judith Burstin
Journal:  Theor Appl Genet       Date:  2022-02-22       Impact factor: 5.699

3.  Fine mapping and candidate gene analysis of qFL-chr1, a fiber length QTL in cotton.

Authors:  Peng Xu; Jin Gao; Zhibin Cao; Peng W Chee; Qi Guo; Zhenzhen Xu; Andrew H Paterson; Xianggui Zhang; Xinlian Shen
Journal:  Theor Appl Genet       Date:  2017-03-30       Impact factor: 5.699

4.  Low validation rate of quantitative trait loci for Gibberella ear rot resistance in European maize.

Authors:  Pedro Correa Brauner; Albrecht E Melchinger; Tobias A Schrag; H Friedrich Utz; Wolfgang Schipprack; Bettina Kessel; Milena Ouzunova; Thomas Miedaner
Journal:  Theor Appl Genet       Date:  2016-10-05       Impact factor: 5.699

5.  SNP discovery and genetic mapping using genotyping by sequencing of whole genome genomic DNA from a pea RIL population.

Authors:  Gilles Boutet; Susete Alves Carvalho; Matthieu Falque; Pierre Peterlongo; Emeline Lhuillier; Olivier Bouchez; Clément Lavaud; Marie-Laure Pilet-Nayel; Nathalie Rivière; Alain Baranger
Journal:  BMC Genomics       Date:  2016-02-18       Impact factor: 3.969

6.  Genome-wide association mapping of partial resistance to Aphanomyces euteiches in pea.

Authors:  Aurore Desgroux; Virginie L'Anthoëne; Martine Roux-Duparque; Jean-Philippe Rivière; Grégoire Aubert; Nadim Tayeh; Anne Moussart; Pierre Mangin; Pierrick Vetel; Christophe Piriou; Rebecca J McGee; Clarice J Coyne; Judith Burstin; Alain Baranger; Maria Manzanares-Dauleux; Virginie Bourion; Marie-Laure Pilet-Nayel
Journal:  BMC Genomics       Date:  2016-02-20       Impact factor: 3.969

Review 7.  Quantitative Resistance to Plant Pathogens in Pyramiding Strategies for Durable Crop Protection.

Authors:  Marie-Laure Pilet-Nayel; Benoît Moury; Valérie Caffier; Josselin Montarry; Marie-Claire Kerlan; Sylvain Fournet; Charles-Eric Durel; Régine Delourme
Journal:  Front Plant Sci       Date:  2017-10-27       Impact factor: 5.753

8.  Mapping QTL associated with partial resistance to Aphanomyces root rot in pea (Pisum sativum L.) using a 13.2 K SNP array and SSR markers.

Authors:  Longfei Wu; Rudolph Fredua-Agyeman; Sheau-Fang Hwang; Kan-Fa Chang; Robert L Conner; Debra L McLaren; Stephen E Strelkov
Journal:  Theor Appl Genet       Date:  2021-06-15       Impact factor: 5.699

9.  Single and multiple resistance QTL delay symptom appearance and slow down root colonization by Aphanomyces euteiches in pea near isogenic lines.

Authors:  C Lavaud; M Baviere; G Le Roy; M R Hervé; A Moussart; R Delourme; M-L Pilet-Nayel
Journal:  BMC Plant Biol       Date:  2016-07-27       Impact factor: 4.215

Review 10.  Genomic Tools in Pea Breeding Programs: Status and Perspectives.

Authors:  Nadim Tayeh; Grégoire Aubert; Marie-Laure Pilet-Nayel; Isabelle Lejeune-Hénaut; Thomas D Warkentin; Judith Burstin
Journal:  Front Plant Sci       Date:  2015-11-27       Impact factor: 5.753
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