BACKGROUND: Effect of administration of Orexin-A into nucleus accumbens (NAcc) in relation to the regulation of feeding behavior and alcohol consumption at specific time intervals is relatively unknown. MATERIALS AND METHODS: In this study, Male Wistar albino rats (n = 54) weighing about 250 ± 10 grams were implanted bilaterally with guide cannula (22 gauze) to target NAcc by stereotaxic surgery. Saline (0.9%) for control and Orexin-A for experimental groups (100 pmol or 250 pmol) were infused by Harvard picoplus pump. Food, water and alcohol (10%) consumption were measured at 1, 2, 4 and 24 hours to evaluate the effect of Orexin-A in fasted rats (24 hours). Preference study was carried out by two bottle choice test. RESULTS: Orexin-A infusion into NAcc showed significant increase in food at 1 hr in all groups compared to controls (p < 0.05) and alcohol (p < 0.02) intake. The changes were dose dependent. There was no noticeable preference or alcohol. CONCLUSIONS FOR: These findings showed that Orexin-A in NAcc could be involved in feeding and drinking but not alcohol preference. The results highlight the effect of Orexin A infusion into NAcc in consummatory behaviour besides other hypothalamic and mesolimbic centres.
BACKGROUND: Effect of administration of Orexin-A into nucleus accumbens (NAcc) in relation to the regulation of feeding behavior and alcohol consumption at specific time intervals is relatively unknown. MATERIALS AND METHODS: In this study, Male Wistar albino rats (n = 54) weighing about 250 ± 10 grams were implanted bilaterally with guide cannula (22 gauze) to target NAcc by stereotaxic surgery. Saline (0.9%) for control and Orexin-A for experimental groups (100 pmol or 250 pmol) were infused by Harvard picoplus pump. Food, water and alcohol (10%) consumption were measured at 1, 2, 4 and 24 hours to evaluate the effect of Orexin-A in fasted rats (24 hours). Preference study was carried out by two bottle choice test. RESULTS:Orexin-A infusion into NAcc showed significant increase in food at 1 hr in all groups compared to controls (p < 0.05) and alcohol (p < 0.02) intake. The changes were dose dependent. There was no noticeable preference or alcohol. CONCLUSIONS FOR: These findings showed that Orexin-A in NAcc could be involved in feeding and drinking but not alcohol preference. The results highlight the effect of Orexin A infusion into NAcc in consummatory behaviour besides other hypothalamic and mesolimbic centres.
Authors: Ozra Dehkordi; Jed E Rose; Martha I Dávila-García; Richard M Millis; Samar Ali Mirzaei; Kebreten F Manaye; Annapurni Jayam-Trouth Journal: J Alcohol Drug Depend Date: 2017-07-20
Authors: Santosh Mayannavar; K S Rashmi; Yalla Durga Rao; Saraswati Yadav; B Ganaraja Journal: Indian J Pharmacol Date: 2016 Jan-Feb Impact factor: 1.200