Kangan Li1, Zhuoli Zhang2, Linfeng Zheng1,2, Hong Liu3, Wei Wei4, Zhiyu Li1, Zhiyan He1, Andrew C Larson2, Guixiang Zhang1. 1. Department of Radiology, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai 200080, China. 2. Department of Radiology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. 3. Department of Gastroenterology and Hepatology, Shanghai First People's Hospital, Shanghai Jiao Tong, University, Shanghai 200080, China. 4. Department of General Surgery, Xiangya Hospital, Xiangya School of Medicine, Central South University, Changsha 410008, China.
Abstract
AIM: To investigate cyclo (Arg-Gly-Asp-D-Phe-Lys) peptide (RGD)-modified PEGylated dendrimer-entrapped gold nanoparticles (PEGylated Au DENPs-RGD) for targeted computed tomography (CT) imaging of breast carcinomas. MATERIALS & METHODS: PEGylated Au DENPs-RGD were synthesized and characterized. Then, the PEGylated Au DENPs-RGD for targeted CT imaging were investigated using the MDA-MB-435 cell line, an integrin-rich breast carcinoma cells, and mice with MDA-MB-435 xenograft tumors. Finally, silver enhancement staining and integrin αvβ3 immunohistochemistry of the tumors were performed. RESULTS: The synthesized PEGylated Au DENPs-RGD were spherical, water dispersible and biocompatible nanoprobes with a gold nanoparticle core size of 2.8 nm. Due to the presence of the Au nanoparticles, the PEGylated Au DENPs-RGD displayed a higher x-ray attenuation intensity than Omnipaque at the same Au or I concentrations. The conjugated RGD ligand can specifically identify and target overexpressed integrin receptors on MDA-MB-435 cells. After intravenous injection, these nanoprobes accumulated in the targeted area of mice with MDA-MB-435 xenograft tumors, which enabled the tumor to be detected by CT imaging. The histological results confirmed the imaging results. CONCLUSION: The PEGylated Au DENPs-RGD can be used as targeted nanoprobes with good biocompatibility for targeted CT imaging and diagnosis of integrin-positive tumors.
AIM: To investigate cyclo (Arg-Gly-Asp-D-Phe-Lys) peptide (RGD)-modified PEGylated dendrimer-entrapped gold nanoparticles (PEGylated Au DENPs-RGD) for targeted computed tomography (CT) imaging of breast carcinomas. MATERIALS & METHODS: PEGylated Au DENPs-RGD were synthesized and characterized. Then, the PEGylated Au DENPs-RGD for targeted CT imaging were investigated using the MDA-MB-435 cell line, an integrin-rich breast carcinoma cells, and mice with MDA-MB-435 xenograft tumors. Finally, silver enhancement staining and integrin αvβ3 immunohistochemistry of the tumors were performed. RESULTS: The synthesized PEGylated Au DENPs-RGD were spherical, water dispersible and biocompatible nanoprobes with a gold nanoparticle core size of 2.8 nm. Due to the presence of the Au nanoparticles, the PEGylated Au DENPs-RGD displayed a higher x-ray attenuation intensity than Omnipaque at the same Au or I concentrations. The conjugated RGD ligand can specifically identify and target overexpressed integrin receptors on MDA-MB-435 cells. After intravenous injection, these nanoprobes accumulated in the targeted area of mice with MDA-MB-435 xenograft tumors, which enabled the tumor to be detected by CT imaging. The histological results confirmed the imaging results. CONCLUSION: The PEGylated Au DENPs-RGD can be used as targeted nanoprobes with good biocompatibility for targeted CT imaging and diagnosis of integrin-positive tumors.
Authors: Jolanta F Kukowska-Latallo; Kimberly A Candido; Zhengyi Cao; Shraddha S Nigavekar; Istvan J Majoros; Thommey P Thomas; Lajos P Balogh; Mohamed K Khan; James R Baker Journal: Cancer Res Date: 2005-06-15 Impact factor: 12.701
Authors: Thommey P Thomas; Istvan J Majoros; Alina Kotlyar; Jolanta F Kukowska-Latallo; Anna Bielinska; Andrzej Myc; James R Baker Journal: J Med Chem Date: 2005-06-02 Impact factor: 7.446
Authors: Xiangyang Shi; Suhe Wang; Sasha Meshinchi; Mary E Van Antwerp; Xiangdong Bi; Inhan Lee; James R Baker Journal: Small Date: 2007-07 Impact factor: 13.281