| Literature DB >> 26212364 |
Makoto Nagai1, Tsutomu Omatsu2, Hiroshi Aoki3, Konosuke Otomaru4, Takehiko Uto5, Motoya Koizumi6, Fujiko Minami-Fukuda7, Hikaru Takai7, Toshiaki Murakami7, Tsuneyuki Masuda8, Hiroshi Yamasato8, Mai Shiokawa3, Shinobu Tsuchiaka2, Yuki Naoi2, Kaori Sano2, Sachiko Okazaki2, Yukie Katayama2, Mami Oba2, Tetsuya Furuya2, Junsuke Shirai2, Tetsuya Mizutani9.
Abstract
A viral metagenomics approach was used to investigate fecal samples of Japanese calves with and without diarrhea. Of the different viral pathogens detected, read counts gave nearly complete astrovirus-related RNA sequences in 15 of the 146 fecal samples collected in three distinct areas (Hokkaido, Ishikawa, and Kagoshima Prefectures) between 2009 and 2015. Due to the lack of genetic information about bovine astroviruses (BoAstVs) in Japan, these sequences were analyzed in this study. Nine of the 15 Japanese BoAstVs were closely related to Chinese BoAstVs and clustered into a lineage (tentatively named lineage 1) in all phylogenetic trees. Three of 15 strains were phylogenetically separate from lineage 1, showing low sequence identities, and clustered instead with an American strain isolated from cattle with respiratory disease (tentatively named lineage 2). Interestingly, two of 15 strains clustered with lineage 1 in the open reading frame (ORF)1a and ORF1b regions, while they clustered with lineage 2 in the ORF2 region. Remarkably, one of 15 strains exhibited low amino acid sequence similarity to other BoAstVs and was clustered separately with porcine astrovirus type 5 in all trees, and ovine astrovirus in the ORF2 region, suggesting past interspecies transmission.Entities:
Mesh：
Year: 2015 PMID： 26212364 DOI： 10.1007/s00705-015-2543-7
Source DB: PubMed Journal: Arch Virol ISSN： 0304-8608 Impact factor: 2.574