Bo Gun Jang1, Hye Sung Kim1, Kyung Ju Kim2, Ye-Young Rhee2, Woo Ho Kim2, Gyeong Hoon Kang2,3. 1. Department of Pathology, Jeju National University Hospital, Jeju, South Korea. 2. Department of Pathology, Seoul National University College of Medicine, Seoul, South Korea. 3. Laboratory of Epigenetics, Cancer Research Institute, Seoul National University College of Medicine, Seoul, South Korea.
Abstract
AIMS: Intestinal stem cell (ISC) markers such as LGR5, ASCL2, EPHB2 and OLFM4, and their clinical implications have been studied extensively in colorectal cancers (CRCs). However, little is known about their expression in precancerous lesions of CRCs. Here, we investigated the expression and distribution of ISC markers in serrated polyps and conventional adenomas. METHODS AND RESULTS: Reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed that all ISC markers were up-regulated significantly in conventional adenomas with low-grade dysplasia (CALGs) compared with other lesions. RNA in-situ hybridization confirmed that CALGs exhibited strong and diffuse expression of all ISC markers, which indicate a stem cell-like phenotype. However, normal colonic mucosa, hyperplastic polyps and sessile serrated adenomas harboured LGR5(+) cells that were confined to the crypt base and demonstrated an organized expression of ISC markers. Notably, in traditional serrated adenomas, expression of LGR5 and ASCL2 was localized to the ectopic crypts as in the normal crypts, but expression of EPHB2 and OLFM4 was distributed in a diffuse manner, which is suggestive of a progenitor-like features. CONCLUSIONS: The expression and distribution profile of ISC markers possibly provides insights into the organization of stem and progenitor-like cells in each type of precancerous lesion of CRC.
AIMS: Intestinal stem cell (ISC) markers such as LGR5, ASCL2, EPHB2 and OLFM4, and their clinical implications have been studied extensively in colorectal cancers (CRCs). However, little is known about their expression in precancerous lesions of CRCs. Here, we investigated the expression and distribution of ISC markers in serrated polyps and conventional adenomas. METHODS AND RESULTS: Reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed that all ISC markers were up-regulated significantly in conventional adenomas with low-grade dysplasia (CALGs) compared with other lesions. RNA in-situ hybridization confirmed that CALGs exhibited strong and diffuse expression of all ISC markers, which indicate a stem cell-like phenotype. However, normal colonic mucosa, hyperplastic polyps and sessile serrated adenomas harboured LGR5(+) cells that were confined to the crypt base and demonstrated an organized expression of ISC markers. Notably, in traditional serrated adenomas, expression of LGR5 and ASCL2 was localized to the ectopic crypts as in the normal crypts, but expression of EPHB2 and OLFM4 was distributed in a diffuse manner, which is suggestive of a progenitor-like features. CONCLUSIONS: The expression and distribution profile of ISC markers possibly provides insights into the organization of stem and progenitor-like cells in each type of precancerous lesion of CRC.
Authors: Bo Gun Jang; Cheol Lee; Hye Sung Kim; Myung Soo Shin; Min Seok Cheon; Jae Wang Kim; Woo Ho Kim Journal: Virchows Arch Date: 2017-01-09 Impact factor: 4.064
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Authors: Michael K Dame; Durga Attili; Shannon D McClintock; Priya H Dedhia; Peter Ouillette; Olaf Hardt; Alana M Chin; Xiang Xue; Julie Laliberte; Erica L Katz; Gina M Newsome; David R Hill; Alyssa J Miller; Yu-Hwai Tsai; David Agorku; Christopher H Altheim; Andreas Bosio; Becky Simon; Linda C Samuelson; Jay A Stoerker; Henry D Appelman; James Varani; Max S Wicha; Dean E Brenner; Yatrik M Shah; Jason R Spence; Justin A Colacino Journal: Development Date: 2018-03-14 Impact factor: 6.862
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