| Literature DB >> 26210810 |
Xiaobo Zhu1, Sihui Liang1, Junjie Yin1, Can Yuan1, Jing Wang1, Weitao Li1, Min He1, Jichun Wang1, Weilan Chen1, Bingtian Ma1, Yuping Wang1, Peng Qin1, Shigui Li1, Xuewei Chen2.
Abstract
DnaJ proteins belong to chaperones of Hsp40 family that ubiquitously participate in various cellular processes. Previous studies have shown chloroplast-targeted DnaJs are involved in the development of chloroplast in some plant species. However, little is known about the function of DnaJs in rice, one of the main staple crops. In this study, we characterized a type I DnaJ protein OsDjA7/8. We found that the gene OsDjA7/8 was expressed in all collected tissues, with a priority in the vigorous growth leaf. Subcellular localization revealed that the protein OsDjA7/8 was mainly distributed in chloroplast. Reduced expression of OsDjA7/8 in rice led to albino lethal at the seedling stage. Transmission electron microscopy observation showed that the chloroplast structures were abnormally developed in the plants silenced for OsDjA7/8. In addition, the transcriptional expression of the genes tightly associated with the development of chloroplast was deeply reduced in the plants silenced for OsDjA7/8. Collectively, our study reveals that OsDjA7/8 encodes a chloroplast-localized protein and is essential for chloroplast development and differentiation in rice.Entities:
Keywords: Albino; Chloroplast development; DnaJ; OsDjA7/8; RNAi; Rice
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Year: 2015 PMID： 26210810 DOI： 10.1016/j.gene.2015.07.067
Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688