Magda Massae Hata Viveiros1, Ricardo Torres Soares2, Michelle Sako Omodei2, Cláudia Aparecida Rainho2, Carlos Roberto Padovani2, Nilson Cruz2, Silvana Artioli Schellini2, Antonio Carlos Lottelli Rodrigues2. 1. From the Graduate Program in General Basis of Surgery (Viveiros), Botucatu Medical School (Omodei), the Department of Genetics (Rainho), and the Department of Biostatistics (Padovani), Biosciences Institute, and the Department of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery (Schellini, Rodrigues), Universidade Estadual Paulista, Botucatu, and the Department of Control and Automation Engineering (Soares, Cruz), Universidade Estadual Paulista, Sorocaba, São Paulo, Brazil. Electronic address: magdahata@yahoo.com. 2. From the Graduate Program in General Basis of Surgery (Viveiros), Botucatu Medical School (Omodei), the Department of Genetics (Rainho), and the Department of Biostatistics (Padovani), Biosciences Institute, and the Department of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery (Schellini, Rodrigues), Universidade Estadual Paulista, Botucatu, and the Department of Control and Automation Engineering (Soares, Cruz), Universidade Estadual Paulista, Sorocaba, São Paulo, Brazil.
Abstract
PURPOSE: To evaluate the adhesion of human lens capsule cells on hydrophilic acrylic intraocular lenses (IOLs) coated with polyethylene glycol (PEG). SETTING: Department of Ophthalmology, Faculty of Medicine, Universidade Estadual Paulista-Botucatu, São Paulo, Brazil. DESIGN: Experimental study. METHODS: Human anterior lens capsules obtained during cataract surgery were cultured and seeded (200 cells/IOLs) in triplicates on the surface of a copolymer comprising hydroxyethyl methacrylate, ethyl methacrylate, and methyl methacrylate IOLs (Loflex) treated or not treated with PEG. After 26 hours, the number of viable adherent cells was estimated by counting in a hemocytometer. RESULTS: The coating of hydrophilic acrylic IOLs with PEG was effective in inhibiting cell adhesion (P < .05). Cells showing 2 distinct morphologic patterns-epithelial and dendritic-like-were observed during the in vitro establishment of the cultures. A tendency toward greater adhesion of dendritic-like cells was observed in untreated IOLs compared with treated IOLs (P = .095). CONCLUSION: Coating hydrophilic acrylic IOLs with PEG was effective in inhibiting cell adhesion. This treatment might play a role in posterior capsule opacification prevention. FINANCIAL DISCLOSURE: No author has a financial or proprietary interest in any material or method mentioned.
PURPOSE: To evaluate the adhesion of human lens capsule cells on hydrophilic acrylic intraocular lenses (IOLs) coated with polyethylene glycol (PEG). SETTING: Department of Ophthalmology, Faculty of Medicine, Universidade Estadual Paulista-Botucatu, São Paulo, Brazil. DESIGN: Experimental study. METHODS:Human anterior lens capsules obtained during cataract surgery were cultured and seeded (200 cells/IOLs) in triplicates on the surface of a copolymer comprising hydroxyethyl methacrylate, ethyl methacrylate, and methyl methacrylate IOLs (Loflex) treated or not treated with PEG. After 26 hours, the number of viable adherent cells was estimated by counting in a hemocytometer. RESULTS: The coating of hydrophilic acrylic IOLs with PEG was effective in inhibiting cell adhesion (P < .05). Cells showing 2 distinct morphologic patterns-epithelial and dendritic-like-were observed during the in vitro establishment of the cultures. A tendency toward greater adhesion of dendritic-like cells was observed in untreated IOLs compared with treated IOLs (P = .095). CONCLUSION: Coating hydrophilic acrylic IOLs with PEG was effective in inhibiting cell adhesion. This treatment might play a role in posterior capsule opacification prevention. FINANCIAL DISCLOSURE: No author has a financial or proprietary interest in any material or method mentioned.