Zhaoqin Zhu1, Yi Liu1, Lei Xu1, Wencai Guan1, Xiaonan Zhang1, Tangkai Qi1, Bisheng Shi1, Zhigang Song1, Xinian Liu1, Yanmin Wan1, Di Tian1, Jing He1, Xiaoling Zhang1, Min Wu1, Hongzhou Lu1, Shuihua Lu1, Zhiyong Zhang1, Zhenghong Yuan2, Yunwen Hu3. 1. Shanghai Public Health Clinical Center, Fudan University, 2901 Caolang Road, Jinshan 201508 Shanghai, China. 2. Shanghai Public Health Clinical Center, Fudan University, 2901 Caolang Road, Jinshan 201508 Shanghai, China; Key Lab of Medical Molecular Virology, School of Basic Medical Science, Fudan University, 138 Yixueyuan Road, Xuhui, 200032 Shanghai, China. Electronic address: zhyuan@shaphc.org. 3. Shanghai Public Health Clinical Center, Fudan University, 2901 Caolang Road, Jinshan 201508 Shanghai, China. Electronic address: ywhu0117@126.com.
Abstract
BACKGROUND: The clinical features of avian-origin influenza virus A (H7N9) virus infection have been extensively characterized, but viral RNA detection in extra-pulmonary samples has seldom been studied. OBJECTIVES: To study shedding of viral RNA in extra pulmonary samples in patients with avian influenza H7N9 infections. STUDY DESIGN: A retrospective study of throat swabs, urine, fecal samples and sera collected sequentially from 18 hospitalized patients with H7N9 infections in Shanghai, China, between April and July in 2013 was conducted. RESULTS: Viral RNA could be detected in urine samples from 17 patients, in fecal samples from 15 and in sera from 14 with a real-time reverse transcription polymerase chain reaction. The median duration of shedding of viral RNA was 19.7 days in throat swabs, 22 days in feces, 21.1 days in urines and 16.2 days in sera, indicating prolonged shedding of viral RNA in feces and urine compared with that in throat swabs. Prolonged duration of viral RNA detection in throat swabs and urine samples was observed in more severe patients. Moreover, in previously reported oseltamivir resistant patients, the NA gene with a 292K mutation could also be detected in their extra-pulmonary as well as in their respiratory samples. CONCLUSION: Our data indicated a high frequency of viral RNA detection in feces, urine and sera in H7N9-infected patients and pointed out the potential risk of transmission.
BACKGROUND: The clinical features of avian-origin influenza virus A (H7N9) virus infection have been extensively characterized, but viral RNA detection in extra-pulmonary samples has seldom been studied. OBJECTIVES: To study shedding of viral RNA in extra pulmonary samples in patients with avian influenza H7N9 infections. STUDY DESIGN: A retrospective study of throat swabs, urine, fecal samples and sera collected sequentially from 18 hospitalized patients with H7N9 infections in Shanghai, China, between April and July in 2013 was conducted. RESULTS: Viral RNA could be detected in urine samples from 17 patients, in fecal samples from 15 and in sera from 14 with a real-time reverse transcription polymerase chain reaction. The median duration of shedding of viral RNA was 19.7 days in throat swabs, 22 days in feces, 21.1 days in urines and 16.2 days in sera, indicating prolonged shedding of viral RNA in feces and urine compared with that in throat swabs. Prolonged duration of viral RNA detection in throat swabs and urine samples was observed in more severe patients. Moreover, in previously reported oseltamivir resistant patients, the NA gene with a 292K mutation could also be detected in their extra-pulmonary as well as in their respiratory samples. CONCLUSION: Our data indicated a high frequency of viral RNA detection in feces, urine and sera in H7N9-infectedpatients and pointed out the potential risk of transmission.
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Authors: Miranda de Graaf; Relja Beck; Simone M Caccio; Birgitta Duim; Pieter LA Fraaij; Françoise S Le Guyader; Marc Lecuit; Jacques Le Pendu; Emmie de Wit; Constance Schultsz Journal: Curr Opin Virol Date: 2016-11-23 Impact factor: 7.090
Authors: Nelson Lee; Bin Cao; Changwen Ke; Hongzhou Lu; Yunwen Hu; Claudia Ha Ting Tam; Ronald Ching Wan Ma; Dawei Guan; Zhaoqin Zhu; Hui Li; Mulei Lin; Rity Y K Wong; Irene M H Yung; Tin-Nok Hung; Kirsty Kwok; Peter Horby; David Shu Cheong Hui; Martin Chi Wai Chan; Paul Kay Sheung Chan Journal: J Infect Dis Date: 2017-07-01 Impact factor: 5.226