Jia Wang1, Batzaya Davaadelger2, Joelle K Salazar3, Robert R Butler4, Jean-François Pombert5, John J Kilbane6, Benjamin C Stark7. 1. Department of Biology, Illinois Institute of Technology, Chicago, IL, 60616, USA. jwang93@hawk.iit.edu. 2. Department of Biology, Illinois Institute of Technology, Chicago, IL, 60616, USA. batzayada@gmail.com. 3. Department of Biology, Illinois Institute of Technology, Chicago, IL, 60616, USA. jkrieger@iit.edu. 4. Department of Biology, Illinois Institute of Technology, Chicago, IL, 60616, USA. rbutler1@hawk.iit.edu. 5. Department of Biology, Illinois Institute of Technology, Chicago, IL, 60616, USA. jpombert@iit.edu. 6. Department of Biology, Illinois Institute of Technology, Chicago, IL, 60616, USA. kilbane@iit.edu. 7. Department of Biology, Illinois Institute of Technology, Chicago, IL, 60616, USA. starkb@iit.edu.
Abstract
OBJECTIVE: To isolate and characterize novel thermophilic bacteria capable of biodesulfurization of petroleum. RESULTS: A culture containing two Paenibacillus spp. (denoted "32O-W" and "32O-Y") was isolated by repeated passage of a soil sample at up to 55 °C in medium containing dibenzothiophene (DBT) as sulfur source. Only 32O-Y metabolized DBT, apparently via the 4S pathway; maximum activity occurred from 40 to 45 °C, with some activity up to at least 50 °C. 32O-W enhanced DBT metabolism by 32O-Y (by 22-74 % at 40-50 °C). With sulfate as sulfur source, 32O-Y and 32O-W grew well up to 58 and 63 °C, respectively. Selection of a mixed culture of 32O-Y and 32O-W at 54 °C increased DBT metabolism 36-42 % from 40 to 45 °C. Genome sequencing identified desulfurization gene homologs in the strains consistent with their desulfurization properties. CONCLUSION: The 32O-Y/32O-W culture may be a useful starting point for development of an improved thermophilic petroleum biodesulfurization process.
OBJECTIVE: To isolate and characterize novel thermophilic bacteria capable of biodesulfurization of petroleum. RESULTS: A culture containing two Paenibacillus spp. (denoted "32O-W" and "32O-Y") was isolated by repeated passage of a soil sample at up to 55 °C in medium containing dibenzothiophene (DBT) as sulfur source. Only 32O-Y metabolized DBT, apparently via the 4S pathway; maximum activity occurred from 40 to 45 °C, with some activity up to at least 50 °C. 32O-W enhanced DBT metabolism by 32O-Y (by 22-74 % at 40-50 °C). With sulfate as sulfur source, 32O-Y and 32O-W grew well up to 58 and 63 °C, respectively. Selection of a mixed culture of 32O-Y and 32O-W at 54 °C increased DBT metabolism 36-42 % from 40 to 45 °C. Genome sequencing identified desulfurization gene homologs in the strains consistent with their desulfurization properties. CONCLUSION: The 32O-Y/32O-W culture may be a useful starting point for development of an improved thermophilic petroleum biodesulfurization process.
Authors: Jia Wang; Robert R Butler; Fan Wu; Jean-François Pombert; John J Kilbane; Benjamin C Stark Journal: PLoS One Date: 2017-01-06 Impact factor: 3.240