BACKGROUND AND AIMS: Liver regeneration (LR) is of crucial importance to patients with acute liver failure, those undergoing live donor liver transplantations or extended liver resections. Effective treatment strategies aimed at accelerating liver regeneration could offer major benefits in these patients. Due to easy accessibility, human adipose-derived mesenchymal stem cells (HADMSC) are an attractive source for regenerative medicine. Herein, we investigated the effect of HADMSC on LR in a murine model. We hypothesized that HADMSC will promote LR. METHODS: Mice were subjected to CCl4-induced acute liver failure (ALF). Animals in the experimental arm were treated with HADMSC prior to CCl4-induced ALF. Liver injury was evaluated using serum levels of alanine aminotransferase (ALT), serum interleukin-6 (IL-6), and histopathology. Liver samples were stained for a specific marker of regeneration, proliferating cell nuclear antigen (PCNA). RESULTS: Histology, serum IL-6, and ALT release revealed that HADMSC treatment attenuated liver injury compared with control animals. In addition, animals treated with HADMSC were observed to have improved survival and increased number of PCNA positive cells on histology when compared with controls. CONCLUSION: HADMSCs represent a potential therapeutic strategy to promote liver regeneration.
BACKGROUND AND AIMS: Liver regeneration (LR) is of crucial importance to patients with acute liver failure, those undergoing live donor liver transplantations or extended liver resections. Effective treatment strategies aimed at accelerating liver regeneration could offer major benefits in these patients. Due to easy accessibility, human adipose-derived mesenchymal stem cells (HADMSC) are an attractive source for regenerative medicine. Herein, we investigated the effect of HADMSC on LR in a murine model. We hypothesized that HADMSC will promote LR. METHODS:Mice were subjected to CCl4-induced acute liver failure (ALF). Animals in the experimental arm were treated with HADMSC prior to CCl4-induced ALF. Liver injury was evaluated using serum levels of alanine aminotransferase (ALT), serum interleukin-6 (IL-6), and histopathology. Liver samples were stained for a specific marker of regeneration, proliferating cell nuclear antigen (PCNA). RESULTS: Histology, serum IL-6, and ALT release revealed that HADMSC treatment attenuated liver injury compared with control animals. In addition, animals treated with HADMSC were observed to have improved survival and increased number of PCNA positive cells on histology when compared with controls. CONCLUSION: HADMSCs represent a potential therapeutic strategy to promote liver regeneration.
Authors: Ioannis G Papanikolaou; Charalambos Katselis; Konstantinos Apostolou; Themistoklis Feretis; Maria Lymperi; Manousos M Konstadoulakis; Apostolos E Papalois; George C Zografos Journal: Stem Cells Int Date: 2017-03-13 Impact factor: 5.443
Authors: Jaeim Lee; Ok-Hee Kim; Sang Chul Lee; Kee-Hwan Kim; Jin Sun Shin; Ha-Eun Hong; Ho Joong Choi; Say-June Kim Journal: Int J Mol Sci Date: 2019-11-08 Impact factor: 5.923