| Literature DB >> 26202280 |
Kaido Kurrikoff1, Kadi-Liis Veiman, Ülo Langel.
Abstract
The method presents most important steps in estimating a CPP-mediated reporter gene delivery in a mouse model. The method is applicable for administrating noncovalent CPP/pDNA complexes via i.v. injection and for analysis of luciferase levels in tissue homogenates. This method could be extended to analyze the delivery of different nucleic acid cargos with other types of delivery vectors. First, a simple method is presented for assessing the stability of complexes in blood after i.v. administration, based on quantitation of a fluorescently labeled nucleic acid. Secondly, a protocol is presented for assessing and analyzing luciferase levels in mouse organs.Entities:
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Year: 2015 PMID： 26202280 DOI： 10.1007/978-1-4939-2806-4_22
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745