Literature DB >> 26202025

Human parenchymal and non-parenchymal liver cell isolation, culture and characterization.

Georg Damm1, Elisa Pfeiffer2, Britta Burkhardt3, Jan Vermehren4, Andreas K Nüssler5, Thomas S Weiss6.   

Abstract

Many reports describing parenchymal liver cell isolation have been published so far. However, recent evidence has clearly demonstrated that non-parenchymal liver cells play an important role in many pathophysiologies of the liver, such as drug-induced liver diseases, inflammation, and the development of liver fibrosis and cirrhosis. In this study, we present an overview of the current methods for isolating and characterizing parenchymal and non-parenchymal liver cells.

Entities:  

Keywords:  Human hepatocytes; Liver cell culture; Liver cell isolation; Non-parenchymal liver cells; Parenchymal liver cells

Year:  2013        PMID： 26202025     DOI： 10.1007/s12072-013-9475-7

Source DB:  PubMed          Journal:  Hepatol Int        ISSN： 1936-0533            Impact factor:   6.047


  34 in total

1.  A highly specific isolation of rat sinusoidal endothelial cells by the immunomagnetic bead method using SE-1 monoclonal antibody.

Authors:  Takuo Tokairin; Yuji Nishikawa; Yuko Doi; Hitoshi Watanabe; Toshiaki Yoshioka; Mu Su; Yasufumi Omori; Katsuhiko Enomoto
Journal:  J Hepatol       Date:  2002-06       Impact factor: 25.083

2.  Glial fibrillary acidic protein--a cell type specific marker for Ito cells in vivo and in vitro.

Authors:  K Neubauer; T Knittel; S Aurisch; P Fellmer; G Ramadori
Journal:  J Hepatol       Date:  1996-06       Impact factor: 25.083

3.  Vascular adhesion protein-1 and ICAM-1 support the adhesion of tumor-infiltrating lymphocytes to tumor endothelium in human hepatocellular carcinoma.

Authors:  K F Yoong; G McNab; S G Hübscher; D H Adams
Journal:  J Immunol       Date:  1998-04-15       Impact factor: 5.422

4.  Human hepatocytes: isolation, culture, and quality procedures.

Authors:  Daniel Knobeloch; Sabrina Ehnert; Lilianna Schyschka; Peter Büchler; Michael Schoenberg; Jörg Kleeff; Wolfgang E Thasler; Natascha C Nussler; Patricio Godoy; Jan Hengstler; Andreas K Nussler
Journal:  Methods Mol Biol       Date:  2012

Review 5.  Recent advances in the isolation of liver cells.

Authors:  G Alpini; J O Phillips; B Vroman; N F LaRusso
Journal:  Hepatology       Date:  1994-08       Impact factor: 17.425

6.  Differential gene expression and functional analysis of pit cells from regenerating rat liver.

Authors:  C S Xu; X G Chen; C F Chang; G P Wang; W B Wang; L X Zhang; Q S Zhu; L Wang
Journal:  Genet Mol Res       Date:  2011-04-19

7.  Development of murine hepatic sinusoidal endothelial cells characterized by the expression of hyaluronan receptors.

Authors:  Hidenori Nonaka; Minoru Tanaka; Kaori Suzuki; Atsushi Miyajima
Journal:  Dev Dyn       Date:  2007-08       Impact factor: 3.780

8.  CXC chemokine ligand 16 promotes integrin-mediated adhesion of liver-infiltrating lymphocytes to cholangiocytes and hepatocytes within the inflamed human liver.

Authors:  Mathis Heydtmann; Patricia F Lalor; J Albertus Eksteen; Stefan G Hübscher; Mike Briskin; David H Adams
Journal:  J Immunol       Date:  2005-01-15       Impact factor: 5.422

Review 9.  Human hepatic sinusoidal endothelial cells can be distinguished by expression of phenotypic markers related to their specialised functions in vivo.

Authors:  P F Lalor; W K Lai; S M Curbishley; S Shetty; D H Adams
Journal:  World J Gastroenterol       Date:  2006-09-14       Impact factor: 5.742

10.  Culture and characterization of sinusoidal endothelial cells isolated from human liver.

Authors:  G W Daneker; S A Lund; S W Caughman; R A Swerlick; A H Fischer; C A Staley; E W Ades
Journal:  In Vitro Cell Dev Biol Anim       Date:  1998-05       Impact factor: 2.723
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  17 in total

1.  Featured Article: Isolation, characterization, and cultivation of human hepatocytes and non-parenchymal liver cells.

Authors:  Elisa Pfeiffer; Victoria Kegel; Katrin Zeilinger; Jan G Hengstler; Andreas K Nüssler; Daniel Seehofer; Georg Damm
Journal:  Exp Biol Med (Maywood)       Date:  2014-11-12

Review 2.  A Pathway to Personalizing Therapy for Metastases Using Liver-on-a-Chip Platforms.

Authors:  A S Khazali; A M Clark; A Wells
Journal:  Stem Cell Rev Rep       Date:  2017-06       Impact factor: 5.739

3.  Tubulin alpha 8 is expressed in hepatic stellate cells and is induced in transformed hepatocytes.

Authors:  Lisa Rein-Fischboeck; Rebekka Pohl; Elisabeth M Haberl; Sebastian Zimny; Maximilian Neumann; Kristina Eisinger; Thomas S Weiss; Sabrina Krautbauer; Christa Buechler
Journal:  Mol Cell Biochem       Date:  2017-01-07       Impact factor: 3.396

Review 4.  Cell sources for in vitro human liver cell culture models.

Authors:  Katrin Zeilinger; Nora Freyer; Georg Damm; Daniel Seehofer; Fanny Knöspel
Journal:  Exp Biol Med (Maywood)       Date:  2016-07-05

Review 5.  Liver 'organ on a chip'.

Authors:  Colin H Beckwitt; Amanda M Clark; Sarah Wheeler; D Lansing Taylor; Donna B Stolz; Linda Griffith; Alan Wells
Journal:  Exp Cell Res       Date:  2017-12-29       Impact factor: 3.905

6.  Annexin A6 protein is downregulated in human hepatocellular carcinoma.

Authors:  Elisabeth M Meier; Lisa Rein-Fischboeck; Rebekka Pohl; Josef Wanninger; Andrew J Hoy; Thomas Grewal; Kristina Eisinger; Sabrina Krautbauer; Gerhard Liebisch; Thomas S Weiss; Christa Buechler
Journal:  Mol Cell Biochem       Date:  2016-06-23       Impact factor: 3.396

7.  Protocol for Isolation of Primary Human Hepatocytes and Corresponding Major Populations of Non-parenchymal Liver Cells.

Authors:  Victoria Kegel; Daniela Deharde; Elisa Pfeiffer; Katrin Zeilinger; Daniel Seehofer; Georg Damm
Journal:  J Vis Exp       Date:  2016-03-30       Impact factor: 1.355

8.  The truncated splice variant of peroxisome proliferator-activated receptor alpha, PPARα-tr, autonomously regulates proliferative and pro-inflammatory genes.

Authors:  Maria Thomas; Christine Bayha; Kathrin Klein; Simon Müller; Thomas S Weiss; Matthias Schwab; Ulrich M Zanger
Journal:  BMC Cancer       Date:  2015-06-30       Impact factor: 4.430

9.  Adiponectin isoforms differentially affect gene expression and the lipidome of primary human hepatocytes.

Authors:  Josef Wanninger; Gerhard Liebisch; Kristina Eisinger; Markus Neumeier; Charalampos Aslanidis; Lisa Voggenreiter; Rebekka Pohl; Thomas S Weiss; Sabrina Krautbauer; Christa Buechler
Journal:  Metabolites       Date:  2014-05-23

10.  In Vitro Model for Hepatotoxicity Studies Based on Primary Human Hepatocyte Cultivation in a Perfused 3D Bioreactor System.

Authors:  Fanny Knöspel; Frank Jacobs; Nora Freyer; Georg Damm; An De Bondt; Ilse van den Wyngaert; Jan Snoeys; Mario Monshouwer; Marco Richter; Nadja Strahl; Daniel Seehofer; Katrin Zeilinger
Journal:  Int J Mol Sci       Date:  2016-04-16       Impact factor: 5.923
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