Mariona Palou1, Juana Sánchez1, Francisco García-Carrizo1, Andreu Palou1, Catalina Picó1. 1. Laboratory of Molecular Biology, Nutrition and Biotechnology (Nutrigenomics), University of the Balearic Islands and CIBER de Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Palma de Mallorca, Balearic Islands, Spain.
Abstract
SCOPE: This study investigates whether pectin supplementation in adult rats can ameliorate age-associated disturbances in peripheral insulin and leptin actions. METHODS AND RESULTS: Seven-month-old male Wistar rats were divided into three groups: control (rats fed ad libitum a standard-diet), pectin (rats fed ad libitum a standard-diet supplemented with 10% pectin), and pair-fed (rats pair-fed to the pectin group). They were sacrificed after 1 month. Pectin and pair-fed rats showed lower body weight gain and food intake than controls and underwent a decrease in leptin levels and an increase in adiponectin levels. Pectin-treated animals, but not pair-fed ones, showed lower body-fat content and HOMA-IR index after dietary intervention. Compared to controls, pectin-treated rats showed a decline in the expression of genes related to energy uptake (WAT) and lipogenesis (WAT and liver), and increased expression levels of lipolysis- and fatty-acid oxidation-related genes (liver). Some of the changes were not evidenced in the pair-fed group. These effects appear to be associated with improved leptin signaling. CONCLUSION: Ten percent pectin supplementation for 1 month in adult rats decreases body-fat content and ameliorates age-related insulin and leptin resistance more intensely than what could be attributed to the decrease in energy intake, overall contributing to better metabolic health.
SCOPE: This study investigates whether pectin supplementation in adult rats can ameliorate age-associated disturbances in peripheral insulin and leptin actions. METHODS AND RESULTS: Seven-month-old male Wistar rats were divided into three groups: control (rats fed ad libitum a standard-diet), pectin (rats fed ad libitum a standard-diet supplemented with 10% pectin), and pair-fed (rats pair-fed to the pectin group). They were sacrificed after 1 month. Pectin and pair-fed rats showed lower body weight gain and food intake than controls and underwent a decrease in leptin levels and an increase in adiponectin levels. Pectin-treated animals, but not pair-fed ones, showed lower body-fat content and HOMA-IR index after dietary intervention. Compared to controls, pectin-treated rats showed a decline in the expression of genes related to energy uptake (WAT) and lipogenesis (WAT and liver), and increased expression levels of lipolysis- and fatty-acid oxidation-related genes (liver). Some of the changes were not evidenced in the pair-fed group. These effects appear to be associated with improved leptin signaling. CONCLUSION: Ten percent pectin supplementation for 1 month in adult rats decreases body-fat content and ameliorates age-related insulin and leptin resistance more intensely than what could be attributed to the decrease in energy intake, overall contributing to better metabolic health.
Authors: Francisco García-Carrizo; Barbara Cannon; Jan Nedergaard; Catalina Picó; Albert Dols; Ana María Rodríguez; Andreu Palou Journal: Int J Obes (Lond) Date: 2019-08-29 Impact factor: 5.095
Authors: Pan Zhou; Yang Zhao; Pan Zhang; Yan Li; Taotao Gui; Jun Wang; Chao Jin; Lianqiang Che; Jian Li; Yan Lin; Shengyu Xu; Bin Feng; Zhengfeng Fang Journal: Front Microbiol Date: 2017-11-17 Impact factor: 5.640
Authors: Allah Rakha; Fakiha Mehak; Muhammad Asim Shabbir; Muhammad Arslan; Muhammad Modassar Ali Nawaz Ranjha; Waqar Ahmed; Claudia Terezia Socol; Alexandru Vasile Rusu; Abdo Hassoun; Rana Muhammad Aadil Journal: Front Nutr Date: 2022-09-20