Said M Hashemi1, David van der Poorten1, Francisco Barrera1,2, Priyanka Bandara1, Ora Lux3, James Kench4, Jacob George5. 1. Storr Liver Unit, Westmead Millennium Institute, University of Sydney at Westmead Hospital, Darcy Road Westmead, Sydney, NSW 2145, Australia. 2. Departamento de Gastroenterología, Pontificia Universidad Católica, Santiago, Chile. 3. Department of Clinical Chemistry, Prince of Wales Hospital, Sydney, Australia. 4. Department of Anatomical Pathology, Royal Prince Alfred Hospital, Sydney, Australia. 5. Storr Liver Unit, Westmead Millennium Institute, University of Sydney at Westmead Hospital, Darcy Road Westmead, Sydney, NSW 2145, Australia. jacob.george@sydney.edu.au.
Abstract
BACKGROUND: Chronic hepatitis C (CHC) infection is associated with insulin resistance and with oxidative stress, but the relationship between the two has not been thoroughly examined. PURPOSE: To evaluate the association between insulin resistance and oxidative stress in CHC patients. METHOD: In 115 CHC patients (68 with genotype 1 and 47 with genotype 3), the relationship between the serum concentration of malondialdehyde (MDA), a marker of oxidative stress and insulin resistance as defined by the homeostasis model (HOMA-IR) was examined. RESULTS: There was no significant difference in MDA levels between genotype 1- and genotype 3-infected subjects (12.882 vs. 12.426 ng/mL, p = 0.2). By univariate analysis, factors associated with HOMA-IR in both genotypes were oxidative stress as measured by MDA (p = 0.002), body mass index (BMI), portal activity, and fibrosis. Genotype-specific differences in HOMA-IR association were steatosis and triglycerides (TG) for genotype 1, and age and glutathione (GSH) for genotype 3. In a stepwise multiple linear regression analysis in both genotypes, MDA was a significant and independent predictor of HOMA-IR (p = 0.04). As expected, BMI and fibrosis were likewise independently correlated to HOMA-IR. In addition, MDA levels were higher (p < 0.001) and GSH levels were lower (p = 0.023) in insulin-resistant subjects compared to their insulin-sensitive counterparts. CONCLUSIONS: It is concluded that in CHC, oxidative stress is an independent predictor of HOMA-IR, irrespective of virus genotype. Further studies on the role of oxidative stress in the development of insulin resistance in CHC are warranted.
BACKGROUND:Chronic hepatitis C (CHC) infection is associated with insulin resistance and with oxidative stress, but the relationship between the two has not been thoroughly examined. PURPOSE: To evaluate the association between insulin resistance and oxidative stress in CHCpatients. METHOD: In 115 CHCpatients (68 with genotype 1 and 47 with genotype 3), the relationship between the serum concentration of malondialdehyde (MDA), a marker of oxidative stress and insulin resistance as defined by the homeostasis model (HOMA-IR) was examined. RESULTS: There was no significant difference in MDA levels between genotype 1- and genotype 3-infected subjects (12.882 vs. 12.426 ng/mL, p = 0.2). By univariate analysis, factors associated with HOMA-IR in both genotypes were oxidative stress as measured by MDA (p = 0.002), body mass index (BMI), portal activity, and fibrosis. Genotype-specific differences in HOMA-IR association were steatosis and triglycerides (TG) for genotype 1, and age and glutathione (GSH) for genotype 3. In a stepwise multiple linear regression analysis in both genotypes, MDA was a significant and independent predictor of HOMA-IR (p = 0.04). As expected, BMI and fibrosis were likewise independently correlated to HOMA-IR. In addition, MDA levels were higher (p < 0.001) and GSH levels were lower (p = 0.023) in insulin-resistant subjects compared to their insulin-sensitive counterparts. CONCLUSIONS: It is concluded that in CHC, oxidative stress is an independent predictor of HOMA-IR, irrespective of virus genotype. Further studies on the role of oxidative stress in the development of insulin resistance in CHC are warranted.
Authors: N De Maria; A Colantoni; S Fagiuoli; G J Liu; B K Rogers; F Farinati; D H Van Thiel; R A Floyd Journal: Free Radic Biol Med Date: 1996 Impact factor: 7.376
Authors: Khaled Thabet; Anastasia Asimakopoulos; Maryam Shojaei; Manuel Romero-Gomez; Alessandra Mangia; William L Irving; Thomas Berg; Gregory J Dore; Henning Grønbæk; David Sheridan; Maria Lorena Abate; Elisabetta Bugianesi; Martin Weltman; Lindsay Mollison; Wendy Cheng; Stephen Riordan; Janett Fischer; Ulrich Spengler; Jacob Nattermann; Ahmed Wahid; Angela Rojas; Rose White; Mark W Douglas; Duncan McLeod; Elizabeth Powell; Christopher Liddle; David van der Poorten; Jacob George; Mohammed Eslam Journal: Nat Commun Date: 2016-09-15 Impact factor: 14.919