OBJECTIVES: Characterization of a urine isolate of daptomycin non-susceptible Enterococcus faecium recovered from a patient with kidney transplantation and no history of daptomycin exposure. METHODS: After isolation in a urine sample, identification of E. faecium was confirmed by amplification of the E. faecium-specific gene encoding D-alanyl-D-alanine ligase (ddl) and daptomycin susceptibility testing was performed by E-test on cation-adjusted Mueller-Hinton agar. In order to determine the genetic bases of daptomycin resistance, the open reading frames of five genes previously associated with daptomycin resistance in enterococci were sequenced. RESULTS: Substitutions in the response regulator LiaR (S19F) and cardiolipin synthase (R218Q) were identified. CONCLUSIONS: To the best of our knowledge, this is the first characterization of emerging daptomycin resistance in E. faecium in a Spanish hospital in the absence of daptomycin exposure and in a renal transplant recipient.
OBJECTIVES: Characterization of a urine isolate of daptomycin non-susceptible Enterococcus faecium recovered from a patient with kidney transplantation and no history of daptomycin exposure. METHODS: After isolation in a urine sample, identification of E. faecium was confirmed by amplification of the E. faecium-specific gene encoding D-alanyl-D-alanine ligase (ddl) and daptomycin susceptibility testing was performed by E-test on cation-adjusted Mueller-Hinton agar. In order to determine the genetic bases of daptomycin resistance, the open reading frames of five genes previously associated with daptomycin resistance in enterococci were sequenced. RESULTS: Substitutions in the response regulator LiaR (S19F) and cardiolipin synthase (R218Q) were identified. CONCLUSIONS: To the best of our knowledge, this is the first characterization of emerging daptomycin resistance in E. faecium in a Spanish hospital in the absence of daptomycin exposure and in a renal transplant recipient.
Authors: O Gasch; M Camoez; M A Domínguez; B Padilla; V Pintado; B Almirante; C Martín; F López-Medrano; E Ruiz de Gopegui; J R Blanco; G García-Pardo; E Calbo; M Montero; A Granados; A Jover; C Dueñas; M Pujol Journal: J Antimicrob Chemother Date: 2013-10-09 Impact factor: 5.790
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Authors: Rosa Gómez-Gil; Maria Pilar Romero-Gómez; Africa García-Arias; M Gallego Ubeda; M Sota Busselo; Ramón Cisterna; Avelino Gutiérrez-Altés; Jesus Mingorance Journal: Diagn Microbiol Infect Dis Date: 2009-08-29 Impact factor: 2.803
Authors: Milya Davlieva; Angel Tovar-Yanez; Kimberly DeBruler; Paul G Leonard; Michael R Zianni; Cesar A Arias; Yousif Shamoo Journal: J Mol Biol Date: 2016-09-23 Impact factor: 5.469