Yuanshun Zhao1, Yonghong Zhang1, Dongdong Lin1, Kang Li1, Chengzeng Yin1, Xiuhong Liu2, Boxun Jin1, Libo Sun1, Jinhua Liu1, Aiying Zhang3, Ning Li4. 1. Beijing You'an Hospital, Capital Medical University, Beijing, China. 2. Beijing Institute of Hepatology, Beijing You'an Hospital, Capital Medical University, Beijing, China. 3. Beijing Institute of Hepatology, Beijing You'an Hospital, Capital Medical University, Beijing, China liningya@ccmu.edu.cn. 4. Beijing You'an Hospital, Capital Medical University, Beijing, China Beijing Institute of Hepatology, Beijing You'an Hospital, Capital Medical University, Beijing, China liningya@ccmu.edu.cn.
Abstract
OBJECTIVES: To develop and evaluate a protein microarray assay with horseradish peroxidase (HRP) chemiluminescence for quantification of α-fetoprotein (AFP) in serum from patients with hepatocellular carcinoma (HCC). METHODS: A protein microarray assay for AFP was developed. Serum was collected from patients with HCC and healthy control subjects. AFP was quantified using protein microarray and enzyme-linked immunosorbent assay (ELISA). RESULTS: Serum AFP concentrations determined via protein microarray were positively correlated (r = 0.973) with those determined via ELISA in patients with HCC (n = 60) and healthy control subjects (n = 30). Protein microarray showed 80% sensitivity and 100% specificity for HCC diagnosis. ELISA had 83.3% sensitivity and 100% specificity. Protein microarray effectively distinguished between patients with HCC and healthy control subjects (area under ROC curve 0.974; 95% CI 0.000, 1.000). CONCLUSION: Protein microarray is a rapid, simple and low-cost alternative to ELISA for detecting AFP in human serum.
OBJECTIVES: To develop and evaluate a protein microarray assay with horseradish peroxidase (HRP) chemiluminescence for quantification of α-fetoprotein (AFP) in serum from patients with hepatocellular carcinoma (HCC). METHODS: A protein microarray assay for AFP was developed. Serum was collected from patients with HCC and healthy control subjects. AFP was quantified using protein microarray and enzyme-linked immunosorbent assay (ELISA). RESULTS: Serum AFP concentrations determined via protein microarray were positively correlated (r = 0.973) with those determined via ELISA in patients with HCC (n = 60) and healthy control subjects (n = 30). Protein microarray showed 80% sensitivity and 100% specificity for HCC diagnosis. ELISA had 83.3% sensitivity and 100% specificity. Protein microarray effectively distinguished between patients with HCC and healthy control subjects (area under ROC curve 0.974; 95% CI 0.000, 1.000). CONCLUSION: Protein microarray is a rapid, simple and low-cost alternative to ELISA for detecting AFP in human serum.
Authors: Aiying Zhang; Chengzeng Yin; Zhenshun Wang; Yonghong Zhang; Yuanshun Zhao; Ang Li; Huanqin Sun; Dongdong Lin; Ning Li Journal: J Int Med Res Date: 2016-11-24 Impact factor: 1.671