Literature DB >> 26194707

De Novo Assembly of Plasmids Using Yeast Recombinational Cloning.

Ameya A Mashruwala1, Jeffrey M Boyd2.   

Abstract

Molecular cloning is a cornerstone of modern biology laboratories. However, traditional cloning can be time-consuming and problematic. We outline herein a method that utilizes the endogenous gap repair system of yeast cells to clone and assemble DNA constructs. This system is simple, cheap, and requires minimal reagents. It can be used for the assembly of both simple (single DNA fragments) and complex (multiple DNA fragments) constructs into plasmids.

Entities:  

Keywords:  Genetics; Homologous recombination; Molecular biology; Molecular cloning; Staphylococcus; Yeast

Mesh：

Year:  2016        PMID： 26194707     DOI： 10.1007/7651_2015_275

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  10 in total

1.  SaeRS Is Responsive to Cellular Respiratory Status and Regulates Fermentative Biofilm Formation in Staphylococcus aureus.

Authors:  Ameya A Mashruwala; Casey M Gries; Tyler D Scherr; Tammy Kielian; Jeffrey M Boyd
Journal:  Infect Immun       Date:  2017-07-19       Impact factor: 3.441

2.  The copBL operon protects Staphylococcus aureus from copper toxicity: CopL is an extracellular membrane-associated copper-binding protein.

Authors:  Zuelay Rosario-Cruz; Alexander Eletsky; Nourhan S Daigham; Hassan Al-Tameemi; G V T Swapna; Peter C Kahn; Thomas Szyperski; Gaetano T Montelione; Jeffrey M Boyd
Journal:  J Biol Chem       Date:  2019-01-17       Impact factor: 5.157

3.  The Suf Iron-Sulfur Cluster Biosynthetic System Is Essential in Staphylococcus aureus, and Decreased Suf Function Results in Global Metabolic Defects and Reduced Survival in Human Neutrophils.

Authors:  Christina A Roberts; Hassan M Al-Tameemi; Ameya A Mashruwala; Zuelay Rosario-Cruz; Unnati Chauhan; William E Sause; Victor J Torres; William J Belden; Jeffrey M Boyd
Journal:  Infect Immun       Date:  2017-05-23       Impact factor: 3.441

4.  Tools, Strains, and Strategies To Effectively Conduct Anaerobic and Aerobic Transcriptional Reporter Screens and Assays in Staphylococcus aureus.

Authors:  Erin E Price; Paulami Rudra; Javiera Norambuena; Franklin Román-Rodríguez; Jeffrey M Boyd
Journal:  Appl Environ Microbiol       Date:  2021-08-18       Impact factor: 4.792

5.  Staphylococcus aureus SufT: an essential iron-sulphur cluster assembly factor in cells experiencing a high-demand for lipoic acid.

Authors:  Ameya A Mashruwala; Christina A Roberts; Shiven Bhatt; Kerrie L May; Ronan K Carroll; Lindsey N Shaw; Jeffrey M Boyd
Journal:  Mol Microbiol       Date:  2016-10-21       Impact factor: 3.501

Review 6.  In vivo DNA assembly using common laboratory bacteria: A re-emerging tool to simplify molecular cloning.

Authors:  Jake F Watson; Javier García-Nafría
Journal:  J Biol Chem       Date:  2019-09-14       Impact factor: 5.157

7.  Staphylococcus aureus lacking a functional MntABC manganese import system has increased resistance to copper.

Authors:  Hassan Al-Tameemi; William N Beavers; Javiera Norambuena; Eric P Skaar; Jeffrey M Boyd
Journal:  Mol Microbiol       Date:  2020-10-25       Impact factor: 3.501

8.  The Staphylococcus aureus SrrAB Regulatory System Modulates Hydrogen Peroxide Resistance Factors, Which Imparts Protection to Aconitase during Aerobic Growth.

Authors:  Ameya A Mashruwala; Jeffrey M Boyd
Journal:  PLoS One       Date:  2017-01-18       Impact factor: 3.240

9.  Impaired respiration elicits SrrAB-dependent programmed cell lysis and biofilm formation in Staphylococcus aureus.

Authors:  Ameya A Mashruwala; Adriana van de Guchte; Jeffrey M Boyd
Journal:  Elife       Date:  2017-02-21       Impact factor: 8.140

10.  The DUF59 Containing Protein SufT Is Involved in the Maturation of Iron-Sulfur (FeS) Proteins during Conditions of High FeS Cofactor Demand in Staphylococcus aureus.

Authors:  Ameya A Mashruwala; Shiven Bhatt; Saroj Poudel; Eric S Boyd; Jeffrey M Boyd
Journal:  PLoS Genet       Date:  2016-08-12       Impact factor: 5.917
  10 in total

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