Isabel Wilches1, Vladimiro Tobar2, Eugenia Peñaherrera1, Nancy Cuzco1, Lourdes Jerves1, Yvan Vander Heyden3, Fabián León-Tamariz1, Elisabet Vila4. 1. Faculty of Chemistry, School of Biochemistry and Pharmacy, University of Cuenca, Cuenca, Ecuador. 2. Faculty of Engineering, University of Cuenca, Cuenca, Ecuador. 3. Department Analytical Chemistry and Pharmaceutical Technology, Vrije Universiteit Brussels-VUB, Belgium. 4. Faculty of Chemistry, School of Biochemistry and Pharmacy, University of Cuenca, Cuenca, Ecuador; Facultat de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain. Electronic address: Elisabet.vila@ucuenca.edu.ec.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Jungia rugosa Less (Asteraceae), popularly known in Ecuador as "Carne humana" or "Fompo", is a vine present into the Andean region. It is traditionally used as medicine for the treatment of bruises, cuts and other external inflammatory processes. This study was designed to investigate the anti-inflammatory activity of J. rugosa leaves extract (JRLE) in rodents. MATERIAL AND METHODS: The acute anti-inflammatory activity was evaluated by animal models, including croton oil-induced ear oedema in mice, carrageenan-induced paw oedema in rats and myeloperoxidase (MPO); the chronic anti-inflammatory activity was evaluated by cotton pellet-induced granuloma. RESULTS: Intraperitoneal administration of JRLE (125, 250, 500mg/kg) significantly (p<0.01-0.001) inhibited the croton oil-induced ear oedema and MPO activity in mice; the carrageenan-induced paw oedema in rats was significantly (p<0.05) reduced by 500mg/kg. Repeated (6 days) administration of the extract to mice previously implanted with cotton pellets reduced the formed granuloma (125mg/kg: 11.7%; 250mg/kg: 17.9%; 500mg/kg: 32.4%) but only the inhibition by 500mg/kg reached statistical significance (p<0.01). CONCLUSIONS: The results show that JRLE is effective as an anti-inflammatory agent in acute and chronic inflammation in mice, supporting its traditional use.
ETHNOPHARMACOLOGICAL RELEVANCE: Jungia rugosa Less (Asteraceae), popularly known in Ecuador as "Carne humana" or "Fompo", is a vine present into the Andean region. It is traditionally used as medicine for the treatment of bruises, cuts and other external inflammatory processes. This study was designed to investigate the anti-inflammatory activity of J. rugosa leaves extract (JRLE) in rodents. MATERIAL AND METHODS: The acute anti-inflammatory activity was evaluated by animal models, including croton oil-induced ear oedema in mice, carrageenan-induced paw oedema in rats and myeloperoxidase (MPO); the chronic anti-inflammatory activity was evaluated by cotton pellet-induced granuloma. RESULTS: Intraperitoneal administration of JRLE (125, 250, 500mg/kg) significantly (p<0.01-0.001) inhibited the croton oil-induced ear oedema and MPO activity in mice; the carrageenan-induced paw oedema in rats was significantly (p<0.05) reduced by 500mg/kg. Repeated (6 days) administration of the extract to mice previously implanted with cotton pellets reduced the formed granuloma (125mg/kg: 11.7%; 250mg/kg: 17.9%; 500mg/kg: 32.4%) but only the inhibition by 500mg/kg reached statistical significance (p<0.01). CONCLUSIONS: The results show that JRLE is effective as an anti-inflammatory agent in acute and chronic inflammation in mice, supporting its traditional use.
Authors: Carlos A Vásquez-Londoño; Luisa F Cubillos-Cuadrado; Andrea C Forero-Ozer; Paola A Escobar-Espinosa; David O Cubillos-López; Daniel F Castaño-Betancur Journal: Adv Exp Med Biol Date: 2021 Impact factor: 2.622
Authors: Rosa H M Silva; Nathália de Fátima M Lima; Alberto J O Lopes; Cleydlenne C Vasconcelos; José W C de Mesquita; Ludmilla S S de Mesquita; Fernando C V M Lima; Maria N de S Ribeiro; Ricardo M Ramos; Maria do Socorro de S Cartágenes; João B S Garcia Journal: Front Pharmacol Date: 2017-05-22 Impact factor: 5.810